Small RNAs in the animal gonad: guarding genomes and guiding development.
Small RNAs in the animal gonad: guarding genomes and guiding development.
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DOI：
10.1016/j.biocel.2010.03.005
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发表时间：
2010-08
影响因子：
4
通讯作者：
Lau, Nelson C.
中科院分区：
文献类型：
--
作者：
Lau, Nelson C.
生殖细胞必须以极高的精度保护、分配、包装和传递其基因组，以确保正确的繁殖和胚胎发育。经典遗传学方法已经鉴定出许多控制动物生殖细胞发育的基因，但直到最近，其中一些基因才与 RNA 干扰 (RNAi) 途径相关联，RNA 干扰是一种以小调节 RNA 为中心的基因沉默机制。生殖细胞含有微小RNA（miRNA）、内源性siRNA（endo-siRNA）和Piwi相互作用RNA（piRNA）；它们与 Piwi/Argonaute 蛋白家族的成员结合。众所周知，piwi 基因可以指定生殖细胞的发育，但我们现在了解到，破坏生殖细胞发育的突变也会影响小 RNA 的积累。生殖细胞中的小RNA研究揭示了调节机制的惊人多样性以及生殖系基因在控制转座元件传播方面的统一功能。未来的挑战将是了解种系小 RNA 的产生并确定这些 RNA 的基因调控的全面范围。这一领域的进展可能会影响操纵干细胞和预防由移动 DNA 元件转座引起的疾病的生物医学目标。
Germ cells must safeguard, apportion, package, and deliver their genomes with exquisite precision to ensure proper reproduction and embryonic development. Classical genetic approaches have identified many genes controlling animal germ cell development, but only recently have some of these genes been linked to the RNA interference (RNAi) pathway, a gene silencing mechanism centered on small regulatory RNAs. Germ cells contain microRNAs (miRNAs), endogenous siRNAs (endo-siRNAs), and Piwi-interacting RNAs (piRNAs); these are bound by members of the Piwi/Argonaute protein family. piwi genes were known to specify germ cell development, but we now understand that mutations disrupting germline development can also affect small RNA accumulation. Small RNA studies in germ cells have revealed a surprising diversity of regulatory mechanisms and a unifying function for germline genes in controlling the spread of transposable elements. Future challenges will be to understand the production of germline small RNAs and to identify the full breadth of gene regulation by these RNAs. Progress in this area will likely impact biomedical goals of manipulating stem cells and preventing diseases caused by the transposition of mobile DNA elements.
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影响因子：
10.5
作者：
Chen, PY;Manninga, H;Tuschl, T
通讯作者：
Tuschl, T
DOI：
10.1073/pnas.0911640106
发表时间：
2009-12-01
影响因子：
11.1
作者：
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通讯作者：
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DOI：
10.1073/pnas.0805943105
发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
10.5
作者：
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通讯作者：
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影响因子：
9.2
作者：
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通讯作者：
Schupbach, Trudi