Analysis of 5' junctions of human LINE-1 and Alu retrotransposons suggests an alternative model for 5'-end attachment requiring microhomology-mediated end-joining.
Analysis of 5' junctions of human LINE-1 and Alu retrotransposons suggests an alternative model for 5'-end attachment requiring microhomology-mediated end-joining.
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对人类 LINE-1 和 Alu 逆转录转座子 5 连接的分析表明，需要微同源介导的末端连接的 5 末端附着的替代模型。
DOI：
10.1101/gr.3421505
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发表时间：
2005
期刊：
影响因子：
7
通讯作者：
Schumann,GeraldG
中科院分区：
文献类型：
--
作者：
Zingler,Nora;Willhoeft,Ute;Brose,Hans-Peter;Schoder,Volker;Jahns,Thomas;Hanschmann,Kay-MartinO;Morrish,TammyA;Lower,Johannes;Schumann,GeraldG
人类非ltr反转录转座子LINE-1 （L1）插入染色体DNA被认为是由一种称为靶引逆转录（TPRT）的机制启动的。这种机制很容易解释L1拷贝的3 ‘端附着到基因组靶标上，但随后的整合步骤导致5 ’端附着到染色体DNA上，这仍然是猜测的原因。通过应用生物信息学分析人类基因组中最近L1插入的5 ‘连接，我们提供了证据，证明L1使用至少两种不同的机制将新生L1拷贝的5 ’端连接到其基因组靶点。虽然5 ‘端截断的L1元件在其靶位点和截断点之间对重叠核苷酸的短片段有统计学上的显著偏好，但全长插入在其5 ’端对这种微同源性没有明显的偏好。在第二种全基因组方法中，我们分析了aluelements，以检查这些被认为通过L1蛋白动员的非自主反转录转座子是否表现出类似的特征。我们发现aluelements似乎主要通过不涉及重叠核苷酸的途径整合。结果表明，细胞非同源DNA末端连接途径可以从不完全L1反转录转位事件中解决中间产物，从而导致5 '截断。
Insertion of the human non-LTR retrotransposon LINE-1 (L1) into chromosomal DNA is thought to be initiated by a mechanism called target-primed reverse transcription (TPRT). This mechanism readily accounts for the attachment of the 3′-end of an L1 copy to the genomic target, but the subsequent integration steps leading to the attachment of the 5′-end to the chromosomal DNA are still cause for speculation. By applying bioinformatics to analyze the 5′ junctions of recent L1 insertions in the human genome, we provide evidence that L1 uses at least two distinct mechanisms to link the 5′-end of the nascent L1 copy to its genomic target. While 5′-truncated L1 elements show a statistically significant preference for short patches of overlapping nucleotides between their target site and the point of truncation, full-length insertions display no distinct bias for such microhomologies at their 5′-ends. In a second genome-wide approach, we analyzedAluelements to examine whether these nonautonomous retrotransposons, which are thought to be mobilized through L1 proteins, show similar characteristics. We found thatAluelements appear to be predominantly integrated via a pathway not involving overlapping nucleotides. The results indicate that a cellular nonhomologous DNA end-joining pathway may resolve intermediates from incomplete L1 retrotransposition events and thus lead to 5′ truncations.
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影响因子：
14.9
作者：
John Thacker;Jeremy Chalk;Anil Ganesh;Phillip North
通讯作者：
John Thacker;Jeremy Chalk;Anil Ganesh;Phillip North
影响因子：
9.8
作者：
Myers, JS;Vincent, BJ;Batzer, MA
通讯作者：
Batzer, MA
影响因子：
11.4
作者：
SCHWARZSOMMER, Z;LECLERCQ, L;SAEDLER, H
通讯作者：
SAEDLER, H
人β-珠蛋白基因3′端附近Kpnl家族成员的序列分析
DOI：
--
发表时间：
1985
期刊：
影响因子：
--
作者：
M. Hattori;S. Hidaka;Y. Sakaki
通讯作者：
Y. Sakaki
DOI：
10.1073/pnas.94.19.10155
发表时间：
1997-09-16
影响因子：
11.1
作者：
Kolosha, VO;Martin, SL
通讯作者：
Martin, SL