Octamer-primed cycle sequencing: design of an optimized primer library.
Octamer-primed cycle sequencing: design of an optimized primer library.
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八聚体引物循环测序：优化引物库的设计。
DOI：
10.1101/gr.6.6.545
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发表时间：
1996
期刊：
影响因子：
7
通讯作者：
McCollum,JC
中科院分区：
文献类型：
--
作者：
Hardin,SH;Jones,LB;Homayouni,R;McCollum,JC
本文介绍了一种利用八聚体寡核苷酸启动DNA测序反应的新方法。八聚体测序与同位素和荧光测序化学兼容，优化了反应条件，使样品可以并行处理，并且该过程具有自动化的潜力。这一策略比传统的引物步行测序策略更快，因为有了引物库，就可以立即获得用于下一步测序反应的引物库，从而消除了设计和合成基因特异性引物所带来的延迟。八聚体文库由优化的测序引物组成，这样八聚体测序产生的结果与传统的引物行走相同或更好。这项技术更经济，因为反应中的主要成本-基因特异性测序引物被能够启动多次反应的频繁出现的八聚体的优化子集所取代。
This paper describes a novel method of primer walking using octamer oligonucleotides to prime DNA sequencing reactions. Octamer sequencing is compatible with isotopic and fluorescent sequencing chemistry, reaction conditions are optimized such that the samples can be processed in parallel, and the procedure has the potential to be automated. This strategy is faster than the traditional primer walking sequencing strategy, as the existence of a primer library allows immediate access to a primer for the next sequencing reaction, eliminating delays associated with designing and synthesizing gene-specific primers. The octamer library is comprised of optimized sequencing primers, such that octamer sequencing yields results equivalent to or better than traditional primer walking. This technology is more economical because gene-specific sequencing primers, the major cost in the reaction, are replaced by an optimized subset of frequently occurring octamers that are able to prime multiple reactions.
包含 3342 个用于基因组测序的有用九聚物引物的文库。
影响因子：
3.5
作者：
D. Siemieniak;J. Slightom
通讯作者：
J. Slightom
影响因子：
4.4
作者：
Junya Toguchida;T. L. McGee;Jennifer C. Paterson;Janine R. Eagle;Stephanie Tucker;D. Yandell;T. Dryja
通讯作者：
Junya Toguchida;T. L. McGee;Jennifer C. Paterson;Janine R. Eagle;Stephanie Tucker;D. Yandell;T. Dryja
DOI：
10.1073/pnas.90.9.4241
发表时间：
1993-05-01
影响因子：
11.1
作者：
KOTLER, LE;ZEVINSONKIN, D;ULANOVSKY, LE
通讯作者：
ULANOVSKY, LE