Cloned origin of DNA replication in c-myc gene can function and be transmitted in transgenic mice in an episomal state.
Cloned origin of DNA replication in c-myc gene can function and be transmitted in transgenic mice in an episomal state.
复制标题
c-myc基因中克隆的DNA复制起点可以在游离状态的转基因小鼠中发挥作用并传播。
DOI：
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发表时间：
1990
影响因子：
14.9
通讯作者：
Hiroyoshi Ariga
中科院分区：
文献类型：
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作者：
Katsuko Sudo;Masaaki Ogata;Yoshinari Sato;Sanae M. M. Iguchi;Hiroyoshi Ariga
c-myc蛋白最近已被证明与一个区域具有假定的DNA复制起点和增强子活动位于2kb的c-myc基因本身的上游相互作用。通过将含有该区域的构建体（称为pmyc（H-P））注射到受精的小鼠卵中获得转基因小鼠。转基因元件能够在所有小鼠组织中有效复制，即使在高度分化的细胞中也能保持游离状态。此外，pmyc（H-P）可在几代中传递给后代，这表明来自c-myc基因上游区域的片段具有质粒在细胞中的分配、稳定性和DNA复制所必需的序列。此外，我们已经证明，质粒可能只被卵子捕获，而不是精子。
The c-myc protein has recently been shown to interact with a region possessing putative origin of DNA replication and enhancer activities located 2 kb upstream of the c-myc gene itself. Transgenic mice were obtained by injecting constructs containing this region, termed pmyc(H-P), into fertilized mouse eggs. The transgenic elements were capable of efficient replication in all mouse tissues examined and were maintained in an episomal state even in highly differentiated cells. Moreover, pmyc(H-P) was transmittable to the progeny throughout several generations, which suggests that the fragment derived from the region upstream of the c-myc gene possesses sequences necessary for partition, stability and DNA replication of the plasmid in the cells. In addition, we have shown that the plasmid might be captured only by eggs, not by sperm.
影响因子：
56.9
作者：
T. Stewart;A. Bellvé;P. Leder
通讯作者：
T. Stewart;A. Bellvé;P. Leder