The antimicrobial activity of zinc against group B Streptococcus is strain-dependent across diverse sequence types, capsular serotypes, and invasive versus colonizing isolates.
The antimicrobial activity of zinc against group B Streptococcus is strain-dependent across diverse sequence types, capsular serotypes, and invasive versus colonizing isolates.
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DOI：
10.1186/s12866-021-02428-3
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发表时间：
2022-01-13
期刊：
影响因子：
4.2
通讯作者：
Gaddy JA
中科院分区：
文献类型：
--
作者：
Francis JD;Guevara MA;Lu J;Madhi SA;Kwatra G;Aronoff DM;Manning SD;Gaddy JA
无乳链球菌或B族链球菌（GBS）是一种被包裹的革兰氏阳性细菌病原体，通常定植于人类宿主的下胃肠道和生殖道。这种细菌可感染妊娠生殖道，引起孕妇和新生儿的侵袭性感染。在生殖道定植后，细菌细胞面临着由宿主施加的许多营养挑战。宿主先天免疫系统采用的一种策略是用某些过渡金属（如锌）使细菌入侵者中毒。先前的研究表明，GBS必须采用优雅的策略来绕过锌胁迫，以便在脊椎动物宿主中生存。我们评估了来自不同分离来源、荚膜血清型和序列型的30株GBS菌株对锌中毒的敏感性或抗性。侵袭性菌株，如从GBS感染的早期发病疾病表现中分离出来的菌株，对锌毒性的敏感性明显低于从孕妇直肠阴道拭子中分离出来的定殖菌株。此外，荚膜III型（cpsIII）菌株和ST-17和ST-19菌株对锌胁迫表现出最大的恢复力，而ST-1和ST-12菌株以及荚膜Ib型（cpsIb）菌株对锌中毒更敏感。因此，本研究表明，过渡金属锌对多种GBS菌株具有抗菌性能，其分离来源、荚膜血清型和序列型对锌胁迫的敏感性或抗性有影响。
Streptococcus agalactiae or Group B Streptococcus (GBS) is an encapsulated gram-positive bacterial pathobiont that commonly colonizes the lower gastrointestinal tract and reproductive tract of human hosts. This bacterium can infect the gravid reproductive tract and cause invasive infections of pregnant patients and neonates. Upon colonizing the reproductive tract, the bacterial cell is presented with numerous nutritional challenges imposed by the host. One strategy employed by the host innate immune system is intoxication of bacterial invaders with certain transition metals such as zinc. Previous work has demonstrated that GBS must employ elegant strategies to circumnavigate zinc stress in order to survive in the vertebrate host. We assessed 30 strains of GBS from diverse isolation sources, capsular serotypes, and sequence types for susceptibility or resistance to zinc intoxication. Invasive strains, such as those isolated from early onset disease manifestations of GBS infection were significantly less susceptible to zinc toxicity than colonizing strains isolated from rectovaginal swabs of pregnant patients. Additionally, capsular type III (cpsIII) strains and the ST-17 and ST-19 strains exhibited the greatest resilience to zinc stress, whereas ST-1 and ST-12 strains as well as those possessing capsular type Ib (cpsIb) were more sensitive to zinc intoxication. Thus, this study demonstrates that the transition metal zinc possesses antimicrobial properties against a wide range of GBS strains, with isolation source, capsular serotype, and sequence type contributing to susceptibility or resistance to zinc stress.
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影响因子：
3
作者：
Andreini, Claudia;Bertini, Ivano;Thornton, Janet M.
通讯作者：
Thornton, Janet M.
影响因子：
11.8
作者：
Manning, Shannon D.;Lewis, Maggi A.;Davies, H. Dele
通讯作者：
Davies, H. Dele
影响因子：
9.4
作者：
Lin, FYC;Whiting, A;Bohnsack, JF
通讯作者：
Bohnsack, JF
影响因子：
30.3
作者：
Botella H;Peyron P;Levillain F;Poincloux R;Poquet Y;Brandli I;Wang C;Tailleux L;Tilleul S;Charrière GM;Waddell SJ;Foti M;Lugo-Villarino G;Gao Q;Maridonneau-Parini I;Butcher PD;Castagnoli PR;Gicquel B;de Chastellier C;Neyrolles O
通讯作者：
Neyrolles O
DOI：
10.1038/nrmicro2836
发表时间：
2012-07-16
期刊：
Nature reviews. Microbiology
影响因子：
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作者：
通讯作者：
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