Infrared studies of interaction between metal ions and Ca2+‐binding proteins Marker bands for identifying the types of coordination of the side‐chain COO− groups to metal ions in pike parvalbumin (pI = 4.10)
Infrared studies of interaction between metal ions and Ca2+‐binding proteins Marker bands for identifying the types of coordination of the side‐chain COO− groups to metal ions in pike parvalbumin (pI = 4.10)
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金属离子和 Ca2+ 结合蛋白之间相互作用的红外研究 用于识别梭子小清蛋白中侧链 COO− 基团与金属离子配位类型的标记带 (pI = 4.10)
DOI：
10.1016/0014-5793(94)00645-8
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
A. Tsutsumi
中科院分区：
文献类型：
--
作者：
M. Nara;M. Tasumi;M. Tanokura;T. Hiraoki;M. Yazawa;A. Tsutsumi
梭子鱼小白蛋白 (pI = 4.10) 的 Ca2+ 结合位点中的金属配体相互作用已通过傅里叶变换红外光谱进行了检查。 COO-反对称拉伸区域提供了关于 COO-基团与 Mg2+-、Mn2+- 和 Ca2+- 结合形式的金属离子的配位类型的有用信息。在Ca2+结合型的光谱中，在1,582和1,553 cm−1处观察到两个带，而在Mg2+和Mn2+结合型的光谱中，仅在1,582 cm−1附近的区域中观察到带，而在1,553 cm−1附近的区域中没有发现带。 Ca2+结合形式的1,553-cm−1带反映了CD位点中的Glu-62和EF位点中的Glu-101的COO-基团与Ca2+离子的双齿配位，这已通过X射线分析明确为Ca2+结合形式的特征。 Mn2+结合形式的光谱中不存在这样的带与该形式的X射线结构一致，其中两个COO-基团都是同一的。这些以 Mn2+ 结合形式存在的 Glu-62 和 Glu-101 的单齿 COO-基团似乎在 1,577-1,574 cm- 处产生一条带。 Mg2+结合形式的光谱也与Mg2+离子仅占据EF位点的X射线结构中报告的Glu-101的COO-基团的“伪桥接”配位一致，并且相同的光谱进一步表明Glu-62的COO-基团的“伪桥接”配位。
Metal‐ligand interactions in the Ca2+‐binding sites of pike parvalbumin (pI = 4.10) have been examined by Fourier‐transform infrared spectroscopy. The region of the COO−antisymmetric stretch provides useful information on the types of coordination of the COO−groups to the metal ions in the Mg2+‐, Mn2+‐, and Ca2+‐bound forms. In the spectrum of the Ca2+‐bound form, two bands are observed at 1,582 and 1,553 cm−, whereas, in the spectra of the Mg2+‐ and Mn2+‐bound forms, bands are observed only in the region around 1,582 cm−1and no band is found in the region around 1,553 cm−1. The 1,553‐cm−1band of the Ca2+‐bound form reflects the bidentate coordination of the COO−groups of both Glu‐62 in the CD site and Glu‐101 in the EF site to the Ca2+ions, which has been made clear by X‐ray analysis as a feature of the Ca2+‐bound form. Absence of such a band in the spectrum of the Mn2+‐bound form is consistent with the X‐ray structure of this form where both of the two COO−groups are unidentate. These unidentate COO−groups of Glu‐62 and Glu‐101 in the Mn2+‐bound form seem to give rise to a band at 1,577‐1,574 cm−. The spectrum of the Mg2+‐bound form is also consistent with the ‘pseudo‐bridging’ coordination of the COO−group of Glu‐101 reported in the X‐ray structure of a form where the Mg2+ion occupies only the EF site, and the same spectrum is further indicative of the ‘pseudo‐bridging’ coordination of the COO−group of Glu‐62.
钙配体鲤鱼小清蛋白 4.25 的精细晶体结构，分辨率为 1.5-A。
影响因子：
2.9
作者：
Kumar,VD;Lee,L;Edwards,BF
通讯作者：
Edwards,BF