Selective activation of antigen-experienced T cells by anti-CD3 constrained on nanoparticles.
Selective activation of antigen-experienced T cells by anti-CD3 constrained on nanoparticles.
复制标题
抗CD3限制了纳米颗粒的抗CD3对抗原经验的T细胞的选择性激活。
DOI：
10.4049/jimmunol.1301433
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发表时间：
2013-11-15
期刊：
影响因子：
--
通讯作者：
Powell JD
中科院分区：
文献类型：
--
作者：
Lo YC;Edidin MA;Powell JD
Activation of T cells through the T cell receptor (TCR) is mediated by the TCR-CD3 signaling complex. Cross linking of this complex with antibodies directed against CD3 leads to potent activation of T cells. However, such activation is not antigen-specific. We exploited the observation that the TCR-CD3 complex is clustered on T cells that have been activated by antigen by using anti-CD3 nanoparticles to selectively activate antigen-experienced mouse T cells. We find that constraining anti-CD3 on the surface of a nanoparticle markedly and selectively enhances proliferation and cytokine production of antigen-experienced T cells but does not activate naïve T cells. This effect was recapitulated in heterogeneous cultures containing mixtures of antigen-specific CD4+ or CD8+ T cells and bystander T cells. Furthermore, in vivo anti-CD3 coated nanoparticles increased the expansion of antigen-specific T cells following vaccination. Overall, these findings indicate that anti-CD3 coated nanoparticles could be use to enhance the efficacy of vaccines and immunotherapy. The results also suggest constraining a ligand on the surface of a nanoparticle might as general strategy for selectively targeting clustered receptors.
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DOI：
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DOI：
10.1073/pnas.1105200108
发表时间：
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影响因子：
11.1
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