Biosurfactant production and characterization of Bacillus sp. ZG0427 isolated from oil-contaminated soil

Biosurfactant production and characterization of Bacillus sp. ZG0427 isolated from oil-contaminated soil
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芽孢杆菌属生物表面活性剂的生产和表征。

DOI:
10.1007/s13213-015-1066-5
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发表时间:
2015-03
影响因子:
3
通讯作者:
Xiuqin Kong
Xiuqin Kong
中科院分区:
生物学4区
文献类型:
--
作者:
Zhi Yang;Bo Qin;Yanlin Li;Xiuqin Kong

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An effective biosurfactant-producing strain, denoted ZG0427, was isolated from oil-contaminated soil samples collected from an oilfield in northwest China using the drop-collapse test, oil displacement test, and surface tension measurements. The strain was identified as Bacillus sp. based on the results of morphological and biochemical tests and 16S rDNA sequence analysis. The kinetics of cell growth and biosurfactant production were monitored. A maximum biosurfactant production of 2.79 g/L was achieved when the cells were cultivated in fermentation medium containing 2 % (w/v) of glucose as the sole carbon source, with shaking (180 rpm) at 37 °C for 32 h. The extracted biosurfactant reduced the surface tension of deionized water to 24.6 mN/m, with a critical micelle concentration of 50 mg/L, and showed a remarkable ability to reduce both surface tension and emulsification over wide ranges of pH (5.0–12.0), temperature (−70 to 121 °C), and NaCl concentrations (0–10 %, w/v), respectively. The biosurfactant was extracted from the culture supernatant and partially characterized as lipopeptide.
DOI: --
发表时间: 2008
期刊: Current Science
影响因子: 1
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