A C. elegans homolog of huntingtin-associated protein 1 is expressed in chemosensory neurons and in a number of other somatic cell types.
A C. elegans homolog of huntingtin-associated protein 1 is expressed in chemosensory neurons and in a number of other somatic cell types.
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DOI：
10.1007/s12031-008-9109-z
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发表时间：
2009-01
影响因子：
3.1
通讯作者：
Gutekunst, Claire-Anne
中科院分区：
文献类型：
--
作者：
Mercer, Kristina B.;Szlam, Sarah M.;Manning, Erin;Gernert, Kim M.;Walthall, Walter W.;Benian, Guy M.;Gutekunst, Claire-Anne
亨廷顿蛋白相关蛋白1（HAP 1）是亨廷顿蛋白的结合伴侣，亨廷顿蛋白是导致亨廷顿病的蛋白质。在哺乳动物中，HAP 1主要存在于大脑中，在神经元中表达。虽然已经为HAP 1提出了几项职能，但其作用尚未明确确定。在这里，我们报告的HAP 1秀丽隐杆线虫同源物称为T27A3.1的鉴定。T27 A3.1与大鼠和人HAP 1以及果蝇HAP 1同源物米尔顿具有保守性。为了确定T27A3.1（多种同种型; a-e）的细胞表达，我们产生了几种表达荧光报告蛋白（GFP和DsRed 2）或在T27A3.1的启动子控制下与GFP融合的全长T27A3.1a/c同种型的转基因蠕虫系。我们已经发现，T27A3.1在许多细胞类型中表达，包括头部和尾部的化学感受神经元的子集。这些包括两栖类化学感觉神经元ASKL和R，ASIL和R，ADFL和ASEL; phasmid神经元PHBL和R;以及蠕虫生存所需的CAN神经元。此外，我们发现T27A3.1a/c的亚细胞定位类似于哺乳动物HAP 1的亚细胞定位，并且T27A3.1a/c定位于柱头样结构。
Huntingtin Associated Protein 1 (HAP1) is a binding partner for huntingtin, the protein responsible for Huntington’s Disease. In mammals, HAP1 is mostly found in brain where it is expressed in neurons. Although several functions have been proposed for HAP1, its role has not yet been clearly established. Here we report on the identification of a HAP1 C.elegans homolog called T27A3.1. T27A3.1 shows conservation with rat and human HAP1 as well as with Milton, a Drosophila HAP1 homolog. To determine the cellular expression of T27A3.1 (multiple isoforms; a-e), we generated several transgenic worm lines expressing a fluorescent reporter protein (GFP and DsRed2) or full length T27A3.1a/c isoforms fused to GFP under the control of the promoter for T27A3.1. We have found that T27A3.1 is expressed in many cell types including a subset of chemosensory neurons in the head and tail. These include the amphid chemosensory neurons ASKL and R, ASIL and R, ADFL and ASEL; the phasmid neurons PHBL and R; and the CAN neurons which are required for worm survival. Furthermore, we show that the subcellular localization of T27A3.1a/c resemble that of mammalian HAP1 and that T27A3.1a/c localize to stigmoid body like structures.
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影响因子：
5.3
作者：
Li, SH;Gutekunst, CA;Li, XJ
通讯作者：
Li, XJ
影响因子：
3.5
作者：
Bertaux, F;Sharp, AH;Wanker, E
通讯作者：
Wanker, E
影响因子：
3.5
作者：
Engelender, S;Sharp, AH;Ross, CA
通讯作者：
Ross, CA
DOI：
10.1073/pnas.92.19.8710
发表时间：
1995-09-12
影响因子：
11.1
作者：
GUTEKUNST, CA;LEVEY, AI;HERSCH, SM
通讯作者：
HERSCH, SM
DOI：
10.1073/pnas.96.1.179
发表时间：
1999-01-05
影响因子：
11.1
作者：
Faber, PW;Alter, JR;Hart, AC
通讯作者：
Hart, AC