Metformin Prevents Renal Stone Formation through an Antioxidant Mechanism In Vitro and In Vivo.
Metformin Prevents Renal Stone Formation through an Antioxidant Mechanism In Vitro and In Vivo.
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二甲双胍通过体外和体内抗氧化机制预防肾结石形成
DOI：
10.1155/2016/4156075
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发表时间：
2016
影响因子：
--
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Yang X;Ding H;Qin Z;Zhang C;Qi S;Zhang H;Yang T;He Z;Yang K;Du E;Liu C;Xu Y;Zhang Z
氧化应激是与肾小管上皮细胞损伤相关的尿石症的致病因素和关键促进因素。本研究旨在探讨二甲双胍对高草酸尿大鼠模型中草酸盐和结石形成引起的肾小管细胞损伤的预防作用。进行 MTT 测定以确定二甲双胍对草酸盐诱导的细胞毒性的保护作用。体外测定细胞内超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平。雄性Sprague-Dawley大鼠分为对照组、乙二醇(EG)治疗组和EG+二甲双胍治疗组。治疗8周后评估肾组织中的氧化应激和晶体形成。二甲双胍显着抑制草酸盐诱导的MDCK细胞和HK-2细胞活力的降低。此外，二甲双胍显着阻止草酸盐诱导的MDCK细胞和HK-2细胞中MDA浓度升高和SOD降低趋势。在体内，与对照组相比，EG治疗组检测到MDA水平增加和SOD活性降低，而这些参数在EG+二甲双胍治疗组中逆转。与EG治疗组相比，EG+二甲双胍治疗组的肾晶体形成显着减少。二甲双胍通过肾小管细胞保护和抗氧化作用抑制尿晶体沉积物的形成。
Oxidative stress is a causal factor and key promoter of urolithiasis associated with renal tubular epithelium cell injury. The present study was designed to investigate the preventive effects of metformin on renal tubular cell injury induced by oxalate and stone formation in a hyperoxaluric rat model. MTT assays were carried out to determine the protection of metformin from oxalate-induced cytotoxicity. The intracellular superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels were measured in vitro. Male Sprague-Dawley rats were divided into control group, ethylene glycol (EG) treated group, and EG + metformin treated group. Oxidative stress and crystal formations were evaluated in renal tissues after 8-week treatment. Metformin significantly inhibited the decrease of the viability in MDCK cells and HK-2 cells induced by oxalate. Besides, metformin markedly prevented the increased concentration of MDA and the decreased tendency of SOD in oxalate-induced MDCK cells and HK-2 cells. In vivo, the increased MDA levels and the reduction of SOD activity were detected in the EG treated group compared with controls, while these parameters reversed in the EG + metformin treated group. Kidney crystal formation in the EG + metformin treated group was decreased significantly compared with the EG treated group. Metformin suppressed urinary crystal deposit formation through renal tubular cell protection and antioxidative effects.
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影响因子：
7.7
作者：
Matveyenko AV;Dry S;Cox HI;Moshtaghian A;Gurlo T;Galasso R;Butler AE;Butler PC
通讯作者：
Butler PC
DOI：
10.1016/j.bbrc.2010.01.119
发表时间：
2010-03-05
影响因子：
3.1
作者：
Piwkowska, Agnieszka;Rogacka, Dorota;Angielski, Stefan
通讯作者：
Angielski, Stefan
影响因子：
--
作者：
Khan, SR
通讯作者：
Khan, SR
影响因子：
6.6
作者：
KUMAR, S;SIGMON, D;MENON, M
通讯作者：
MENON, M
影响因子：
2.2
作者：
Ishibashi, Y.;Matsui, T.;Yamagishi, S.
通讯作者：
Yamagishi, S.