A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors.
A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors.
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DOI：
10.1186/1472-6750-9-81
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发表时间：
2009-09-18
影响因子：
3.5
通讯作者：
Gandrillon O
中科院分区：
文献类型：
--
作者：
Mejia-Pous C;Viñuelas J;Faure C;Koszela J;Kawakami K;Takahashi Y;Gandrillon O
稳定的转基因是理解任何遗传系统不可否认的关键。基于逆转录病毒的插入策略在使用时存在一些技术挑战，通常仅限于一种特定的物种，有时甚至仅限于一种特定的细胞类型，因为感染取决于某些细胞受体。在处理与逆转录病毒策略不相容的细胞模型时，需要一个通用的系统，该系统既可以实现独立于细胞类型的稳定转基因表达，又可以对转染细胞进行有效分选。我们在此报告基于 Tol2 转座子系统的稳定插入转基因技术与磁性细胞分选 (MACS) 技术的结合，该技术允许以高效、可靠和快速的方式特异性选择携带转基因的细胞。这种新的 Tol2/MACS 系统可在原代鸡红细胞培养物中稳定表达，这些细胞高度富集表达目的转基因的细胞。该系统可用于多种脊椎动物物种。
Stable transgenesis is an undeniable key to understanding any genetic system. Retrovirus-based insertional strategies, which feature several technical challenges when they are used, are often limited to one particular species, and even sometimes to a particular cell type as the infection depends on certain cellular receptors. A universal-like system, which would allow both stable transgene expression independent of the cell type and an efficient sorting of transfected cells, is required when handling cellular models that are incompatible with retroviral strategies. We report here on the combination of a stable insertional transgenesis technique, based on the Tol2 transposon system together with the magnetic cell sorting (MACS) technique, which allows specific selection of cells carrying the transgene in an efficient, reliable and rapid way. This new Tol2/MACS system leads to stable expression in a culture of primary chicken erythroid cells highly enriched in cells expressing the transgene of interest. This system could be used in a wide variety of vertebrate species.
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影响因子：
12.4
作者：
Mizuguchi, H;Xu, ZL;Hayakawa, T
通讯作者：
Hayakawa, T
DOI：
10.1073/pnas.97.21.11403
发表时间：
2000-10-10
影响因子：
11.1
作者：
Kawakami, K;Shima, A;Kawakami, N
通讯作者：
Kawakami, N
影响因子：
2.7
作者：
Sato, Yuki;Kasai, Toshiharu;Takahashi, Yoshiko
通讯作者：
Takahashi, Yoshiko
影响因子：
4.4
作者：
Bresson, Corinne;Keime, Celine;Gonin-Giraud, Sandrine
通讯作者：
Gonin-Giraud, Sandrine
影响因子：
46.9
作者：
Sherman, A;Dawson, A;Sang, H
通讯作者：
Sang, H