Synthesis of silver nanoparticles and its contribution to the capability of Bacillus subtilis to deal with polluted waters
Synthesis of silver nanoparticles and its contribution to the capability of Bacillus subtilis to deal with polluted waters
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纳米银的合成及其对枯草芽孢杆菌处理污染水体能力的贡献
DOI：
10.1007/s00253-019-09880-2
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发表时间：
2019-05
影响因子：
5
通讯作者：
Shi Junling
中科院分区：
文献类型：
--
作者：
Zhao Xixi;Yan Lu;Xu Xiaoguang;Zhao Haobin;Lu Yao;Wang Yan;Jiang Chunmei;Shao Dongyan;Zhu Jing;Shi Junling
枯草芽孢杆菌广泛存在于环境中，表现出通过吸附或生物氧化还原处理污染水体中重金属和染料的能力。脂肽在枯草芽孢杆菌这种能力中的作用尚不清楚。本研究发现枯草芽孢杆菌产生的脂肽可以将银离子还原为银纳米粒子（AgNPs），并确定iturin是主要的有效组分。此外，合成的AgNPs成功地用于催化有机染料的还原和水中Pb2+的污染。通过紫外可见光谱、动态光散射、高分辨率透射电子显微镜（HR-TEM）和选择区域电子衍射（SAED）分析，证实了AgNPs的形成。形成的AgNPs呈结晶状，尺寸小（~ 20 nm），呈球形。紫外辐照能显著促进AgNPs的生物合成。pH值为10时，AgNPs的形成速率最高，pH值为9.2时，AgNPs的稳定性最高。在机制上，酪氨酸和多肽是iturin-A中通过Ar-OH基团形成AgNPs的主要基团。研究表明，iturin在枯草芽孢杆菌处理污水的能力中发挥了重要作用，通过合成AgNPs，催化有机染料的还原，减少Pb2+的污染。
Bacillus subtilis widely exists in environment and shows a capability to deal with heavy metals and dyes in polluted waters by adsorption or biological oxidation and reduction. Little is known about the roles of lipopeptides in this capability of B. subtilis. In this study, we found that the lipopeptides produced by B. subtilis could reduce silver ions to silver nanoparticles (AgNPs) and iturin was identified as the major effective fraction. Furthermore, the synthesized AgNPs was successfully used to catalyze the reduction of organic dyes and reduce Pb2+contamination in water. The formation of AgNPs was confirmed by the features analyzed by UV-vis spectroscopy, dynamic light scattering, high-resolution transmission electron microscopy (HR-TEM), and selected area electron diffraction (SAED). The formed AgNPs showed crystalline, with small size (~ 20 nm) and spherical shape. The biosynthesis of AgNPs was significantly accelerated by UV irradiation. A pH of 10 resulted in the highest formation rate, while pH 9.2 provided the most stability of AgNPs. In mechanisms, tyrosine and the polypeptide were identified as the major groups in iturin-A to form AgNPs via Ar-OH groups. The study revealed that iturin played important roles for the capability of B. subtilis to treat polluted water via a possible way by synthesizing AgNPs and then catalyzing the reduction of organic dyes and reducing the contamination of Pb2+.
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影响因子：
5.8
作者：
Annadhasan, M.;SankarBabu, V. R.;Rajendiran, N.
通讯作者：
Rajendiran, N.
影响因子：
2.8
作者：
Pathak, Khyati V.;Keharia, Hareshkumar
通讯作者：
Keharia, Hareshkumar
DOI：
10.1016/j.saa.2017.02.024
发表时间：
2017-05
期刊：
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
影响因子：
--
作者：
K. Muthu;S. Priya
通讯作者：
K. Muthu;S. Priya
影响因子：
6.2
作者：
M. Tajbakhsh;H. Alinezhad;M. Nasrollahzadeh;Taghi A. Kamali
通讯作者：
M. Tajbakhsh;H. Alinezhad;M. Nasrollahzadeh;Taghi A. Kamali
DOI：
10.1063/1.2348868
发表时间：
2006-10
期刊：
The Journal of chemical physics
影响因子：
--
作者：
P. Sherin;O. Snytnikova;Y. Tsentalovich;R. Sagdeev
通讯作者：
P. Sherin;O. Snytnikova;Y. Tsentalovich;R. Sagdeev