Production, crystallization and X-ray diffraction analysis of the protease CT441 from Chlamydia trachomatis.
Production, crystallization and X-ray diffraction analysis of the protease CT441 from Chlamydia trachomatis.
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沙眼衣原体蛋白酶 CT441 的制备、结晶和 X 射线衍射分析
DOI：
10.1107/s2053230x15020518
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hansen G
中科院分区：
文献类型：
--
作者：
Kohlmann F;Shima K;Rupp J;Solbach W;Hilgenfeld R;Hansen G
原核专性细胞内病原体沙眼衣原体是可预防性失明的最常见原因，影响全球约 600 万人。此外，沙眼衣原体是欧洲和美国最常见的性传播病原体，可引起盆腔炎、宫外孕和不孕症。与其他细胞内病原体一样，蛋白酶在衣原体复杂生命周期的大多数阶段发挥着至关重要的作用。 CT441 是一种衣原体蛋白酶，据报道它会干扰宿主细胞的雌激素信号传导。在此，描述了 CT441 无活性变体的重组生产、纯化和结晶，命名为 CT441°（活性位点 Ser455 被 Ala 取代）。 CT441°在空间群P22121中结晶，晶胞参数a = 86.7，b = 184.0，c = 209.6 Å。收集了分辨率为 2.95 Å 的完整衍射数据集。
The prokaryotic obligate intracellular pathogen Chlamydia trachomatis is the most prevalent cause of preventable blindness, affecting approximately six million people worldwide. In addition, C. trachomatis is the most commonly reported sexually transmitted pathogen in Europe and the US, causing pelvic inflammation, ectopic pregnancy and infertility. As in other intracellular pathogens, proteases play crucial roles during most stages of the complex life cycle of Chlamydia. CT441 is a chlamydial protease that has been reported to interfere with oestrogen signalling of the host cell. Here, the recombinant production, purification and crystallization of an inactive variant of CT441, designated CT441° (active-site Ser455 replaced by Ala), are described. CT441° was crystallized in space group P22121, with unit-cell parameters a = 86.7, b = 184.0, c = 209.6 Å. A complete diffraction data set was collected to a resolution of 2.95 Å.
影响因子：
14.9
作者：
Marchler-Bauer A;Lu S;Anderson JB;Chitsaz F;Derbyshire MK;DeWeese-Scott C;Fong JH;Geer LY;Geer RC;Gonzales NR;Gwadz M;Hurwitz DI;Jackson JD;Ke Z;Lanczycki CJ;Lu F;Marchler GH;Mullokandov M;Omelchenko MV;Robertson CL;Song JS;Thanki N;Yamashita RA;Zhang D;Zhang N;Zheng C;Bryant SH
通讯作者：
Bryant SH
影响因子：
3.2
作者：
Kohlmann, Friedrich;Shima, Kensuke;Hansen, Guido
通讯作者：
Hansen, Guido