Toward an understanding of the molecular mechanisms of barnacle larval settlement: a comparative transcriptomic approach.
Toward an understanding of the molecular mechanisms of barnacle larval settlement: a comparative transcriptomic approach.
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DOI：
10.1371/journal.pone.0022913
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Qian PY
中科院分区：
文献类型：
--
作者：
Chen ZF;Matsumura K;Wang H;Arellano SM;Yan X;Alam I;Archer JA;Bajic VB;Qian PY
藤壶藤壶是一种全球性分布的生物污损生物，也是潮间带生态学和幼虫定居研究的模式物种。然而，缺乏基因组信息阻碍了全面阐明其幼虫定居的分子机制。基于焦磷酸测序的转录组学方法被认为是有用的，以确定幼虫定居过程中的关键分子变化。使用454焦磷酸测序，我们总共收集了630，845个读段，其中215，308个来自幼虫阶段，415，537个来自成虫;产生了23，451个重叠群，而77，785个保留为单例。我们注释了92，322个预测的开放阅读框中的31，720个，其匹配NCBI NR数据库中的命中，并鉴定了7，954个在幼虫和成虫阶段之间差异表达的推定基因。通过实时荧光定量PCR和原位杂交技术对其中几个基因进行了进一步的分析，发现：1）卵黄蛋白原在无节幼体晚期有独特的表达，提示其可能是随后的非摄食期的能量来源; 2）甘露糖受体的定位提示其可能参与了鲤的感觉系统; 3）20 kDa-胶浆蛋白同源物在鲤的胶浆腺中表达，可能在附着过程中起作用; 4）受体酪氨酸激酶在鲤阶段表达较高，可能参与幼虫附着过程中的信号感知。我们的研究结果不仅提供了几个新的假设在幼虫定居过程中的基因功能的基础上，但在B这个大的转录组数据集的可用性。amphitrite，以进一步探索这一重要海洋物种的幼虫定居和发育途径。
The barnacle Balanus amphitrite is a globally distributed biofouler and a model species in intertidal ecology and larval settlement studies. However, a lack of genomic information has hindered the comprehensive elucidation of the molecular mechanisms coordinating its larval settlement. The pyrosequencing-based transcriptomic approach is thought to be useful to identify key molecular changes during larval settlement. Using 454 pyrosequencing, we collected totally 630,845 reads including 215,308 from the larval stages and 415,537 from the adults; 23,451 contigs were generated while 77,785 remained as singletons. We annotated 31,720 of the 92,322 predicted open reading frames, which matched hits in the NCBI NR database, and identified 7,954 putative genes that were differentially expressed between the larval and adult stages. Of these, several genes were further characterized with quantitative real-time PCR and in situ hybridization, revealing some key findings: 1) vitellogenin was uniquely expressed in late nauplius stage, suggesting it may be an energy source for the subsequent non-feeding cyprid stage; 2) the locations of mannose receptors suggested they may be involved in the sensory system of cyprids; 3) 20 kDa-cement protein homologues were expressed in the cyprid cement gland and probably function during attachment; and 4) receptor tyrosine kinases were expressed higher in cyprid stage and may be involved in signal perception during larval settlement. Our results provide not only the basis of several new hypotheses about gene functions during larval settlement, but also the availability of this large transcriptome dataset in B. amphitrite for further exploration of larval settlement and developmental pathways in this important marine species.
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影响因子：
1.6
作者：
Kamino, K;Odo, S;Maruyama, T
通讯作者：
Maruyama, T
影响因子：
3.6
作者：
Lind, Ulrika;Rosenblad, Magnus Alm;Blomberg, Anders
通讯作者：
Blomberg, Anders
影响因子：
2.2
作者：
DAVIS, RE;KELLY, TJ;BORKOVEC, AB
通讯作者：
BORKOVEC, AB
影响因子：
1.1
作者：
GLENNER, H;HOEG, JT
通讯作者：
HOEG, JT
DOI：
10.1016/j.jembe.2010.04.012
发表时间：
2010-08-31
影响因子：
2
作者：
Anil, Arga Chandrashekar;Khandeparker, Lidita;Gaonkar, Chetan A.
通讯作者：
Gaonkar, Chetan A.