A genome-wide metabolomic resource for tomato fruit from Solanum pennellii.
A genome-wide metabolomic resource for tomato fruit from Solanum pennellii.
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DOI：
10.1038/srep03859
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发表时间：
2014-01-24
影响因子：
4.6
通讯作者：
Fraser PD
中科院分区：
文献类型：
--
作者：
Perez-Fons L;Wells T;Corol DI;Ward JL;Gerrish C;Beale MH;Seymour GB;Bramley PM;Fraser PD
番茄及其加工产品是最广泛消费的水果之一。然而，它的驯化导致了野生近缘种95%的遗传和化学多样性的丧失。为了阐明这种多样性，开发其植物育种的潜力，以及了解其生物学意义，已经开发了分析方法，同时生产野生近缘种与商业品种的遗传杂交。在这篇文章中，我们描述了一个多平台的代谢组学分析，使用NMR，质谱和HPLC，的渐渗系的番茄与驯化线，以分析和量化等位基因（QTL）负责代谢性状。我们已经确定了与健康相关的抗氧化剂类胡萝卜素和生育酚的QTL，以及约2000种化合物的分子特征。相关性分析揭示了质体中类异戊二烯形成的复杂相互作用，可以外推到其他作物植物。
Tomato and its processed products are one of the most widely consumed fruits. Its domestication, however, has resulted in the loss of some 95% of the genetic and chemical diversity of wild relatives. In order to elucidate this diversity, exploit its potential for plant breeding, as well as understand its biological significance, analytical approaches have been developed, alongside the production of genetic crosses of wild relatives with commercial varieties. In this article, we describe a multi-platform metabolomic analysis, using NMR, mass spectrometry and HPLC, of introgression lines of Solanum pennellii with a domesticated line in order to analyse and quantify alleles (QTL) responsible for metabolic traits. We have identified QTL for health-related antioxidant carotenoids and tocopherols, as well as molecular signatures for some 2000 compounds. Correlation analyses have revealed intricate interactions in isoprenoid formation in the plastid that can be extrapolated to other crop plants.
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作者：
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通讯作者：
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