Functional Scaffolding for Brain Implants: Engineered Neuronal Network by Microfabrication and iPSC Technology
Functional Scaffolding for Brain Implants: Engineered Neuronal Network by Microfabrication and iPSC Technology
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大脑植入物的功能支架：通过微加工和 iPSC 技术设计的神经元网络
DOI：
10.3389/fnins.2019.00890
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发表时间：
2019
影响因子：
4.3
通讯作者：
A. Pimashkin
中科院分区：
文献类型：
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作者：
K. Shimba;Chih;Takahiro Asahina;Fumika Moriya;K. Kotani;Y. Jimbo;A. Gladkov;O. Antipova;Y. Pigareva;V. Kolpakov;I. Mukhina;V. Kazantsev;A. Pimashkin
神经工程方法可以有效地用于设计治疗神经创伤、缺血或神经退行性疾病引起的中枢神经系统和脑损伤的新方法。在过去的十年中，在植入物（支架）开发领域取得了显著的成果，使用各种生物相容性和可生物降解的材料携带神经元细胞植入脑损伤部位以修复其功能。来源于动物或人诱导多能干细胞（iPS）的神经元有望成为理想的细胞来源，并且已经积极研究了用于特定细胞类型的诱导方法以提高功效和特异性。神经再生的关键目标是损伤部位的结构和功能恢复。目标治疗区域具有异质和复杂的网络拓扑结构，其中各种类型的细胞需要用类似的神经元网络结构恢复以恢复正确的功能。然而，目前用于脑植入物的基于支架的技术以均匀的神经元细胞分布进行操作，这限制了脑损伤区域的恢复，并阻止恢复到完全功能的生物组织。在这项研究中，我们提出了一个神经工程的概念，设计一个神经回路与预定义的单向网络架构，提供了一个平衡的兴奋/抑制的支架，形成组织类似的受伤地区使用各种类型的iPS细胞。这样的组织将模仿受伤部位周围的小生境，并将在形态学和拓扑学上整合到大脑中，恢复失去的功能。
Neuroengineering methods can be effectively used in the design of new approaches to treat central nervous system and brain injury caused by neurotrauma, ischemia, or neurodegenerative disorders. During the last decade, significant results were achieved in the field of implant (scaffold) development using various biocompatible and biodegradable materials carrying neuronal cells for implantation into the injury site of the brain to repair its function. Neurons derived from animal or human induced pluripotent stem (iPS) cells are expected to be an ideal cell source, and induction methods for specific cell types have been actively studied to improve efficacy and specificity. A critical goal of neuro-regeneration is structural and functional restoration of the injury site. The target treatment area has heterogeneous and complex network topology with various types of cells that need to be restored with similar neuronal network structure to recover correct functionality. However, current scaffold-based technology for brain implants operates with homogeneous neuronal cell distribution, which limits recovery in the damaged area of the brain and prevents a return to fully functional biological tissue. In this study, we present a neuroengineering concept for designing a neural circuit with a pre-defined unidirectional network architecture that provides a balance of excitation/inhibition in the scaffold to form tissue similar to that in the injured area using various types of iPS cells. Such tissue will mimic the surrounding niche in the injured site and will morphologically and topologically integrate into the brain, recovering lost function.
DOI：
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发表时间：
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期刊：
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影响因子：
--
作者：
D. Wagenaar;R. Madhavan;J. Pine;Steve M. Potter
通讯作者：
D. Wagenaar;R. Madhavan;J. Pine;Steve M. Potter
影响因子：
9.9
作者：
Chen F;Hableel G;Zhao ER;Jokerst JV
通讯作者：
Jokerst JV
影响因子：
2.5
作者：
Bao, WL;Wu, JY
通讯作者：
Wu, JY