Real-time and non-invasive quantitative phase imaging of pancreatic ductal adenocarcinoma cell mechanical properties
Real-time and non-invasive quantitative phase imaging of pancreatic ductal adenocarcinoma cell mechanical properties
复制标题
胰腺导管腺癌细胞机械特性的实时、无创定量相位成像
DOI：
10.1117/12.2508436
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Gillies D
中科院分区：
文献类型：
--
作者：
Gillies D
已知细胞机械特性会影响其细胞和亚细胞功能。虽然原子力显微镜 (AFM) 等评估细胞力学的方法已经可用，但人们越来越需要以无标记和非接触模式测量细胞力学特性，以允许长时间监测细胞行为，并能够测量嵌入细胞外基质中的细胞。在这项研究中，我们采用数字全息显微镜（DHM）结合良好控制的静水压力应用，以非接触方式实时研究细胞机械特性。接种后 24 小时，通过微流体泵对 25cm2 培养瓶内的胰腺导管腺癌粘着激酶 (FAK) 敲除细胞 (Panc47-1 -/-null) 及其相应的重新表达克隆群 (Panc47-1 +/+ 野生型) 进行非破坏性和非侵入性的循环应激。成功地将循环应力直接施加到细胞上，并在纳米尺度上实时记录细胞相应的体积变化，从而得出细胞的力学性能。刺激的幅度和/或频率的变化被转化为相应的细胞反应。在所研究的细胞系之间观察到相对应变率的差异。我们描述了一种以单细胞分辨率实时、非破坏性地对活细胞进行光学弹性成像的新方法。这使得可以长期监测细胞增殖和分化以及疾病进展过程中的机械特性。这可能与细胞的生物力学特性直接相关。
Cellular mechanical properties are known to influence both their cellular and subcellular functions. Whilst methods to assess cellular mechanics such as Atomic Force Microscopy (AFM) are already available, there is an emerging need to measure cellular mechanical properties in a label-free and contactless mode, to allow for long time monitoring of cell behaviour, and to enable measurements of cells embedded in extracellular matrix. In this study, we have employed Digital Holographic Microscopy (DHM) combined with the well-controlled application of hydrostatic pressure to study cellular mechanical properties in real-time and in a noncontact manner. Cyclic stress was applied non-destructively and non-invasively to pancreatic ductal adenocarcinoma Focal Adhesion Kinase (FAK) knockout cells (Panc47-1 -/-null) and their corresponding re-expressing clonal population (Panc47-1 +/+ wild type) within a 25cm2culture flask by a microfluidic pump 24h after seeding. Cyclic stress was successfully applied directly to cells, and corresponding change in volume was recorded in real-time at the nanometre scale for cell, yielding the mechanical properties of the cells. Change in amplitude and/or frequency of the stimuli was translated to corresponding cell response. Differences were observed in relative strain rates between the cell lines under investigation. We have described a novel method to perform optical elastography on live cells at single cell resolution in realtime and non-destructively. This allows for long-term monitoring of mechanical properties during cell proliferation and differentiation, and disease progress. This can be directly related to the biomechanical properties of cells.
影响因子：
3.4
作者：
Kennedy, Brendan F.;McLaughlin, Robert A.;Sampson, David D.
通讯作者：
Sampson, David D.
全息术、研究和技术
DOI：
--
发表时间：
2011
期刊：
影响因子：
--
作者：
Joseph Rosen
通讯作者：
Joseph Rosen
影响因子：
14
作者：
Yim, Evelyn K. F.;Darling, Eric M.;Kulangara, Karina;Guilak, Farshid;Leong, Kam W.
通讯作者：
Leong, Kam W.