Crosstalk Pathway between Trehalose Metabolism and Cytokinin Degradation for the Determination of the Number of Berries per Bunch in Grapes.
Crosstalk Pathway between Trehalose Metabolism and Cytokinin Degradation for the Determination of the Number of Berries per Bunch in Grapes.
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DOI：
10.3390/cells9112378
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发表时间：
2020-10-29
期刊：
影响因子：
6
通讯作者：
Suzuki S
中科院分区：
文献类型：
--
作者：
Moriyama A;Yamaguchi C;Enoki S;Aoki Y;Suzuki S
在葡萄中，每个花序的花朵数量决定了葡萄串的紧密度。具有紧密串和薄皮浆果的葡萄品种很容易发生浆果分裂，特别是在葡萄藤季节降雨量大的种植区，如日本。我们在此报告，葡萄细胞分裂素氧化酶/脱氢酶5（VvCKX 5）决定了葡萄每花序浆果的数量。每束浆果的数量与所测试的品种中的幼花序中的VvCKX 5表达水平成反比。VvCKX 5的过表达显著降低了拟南芥中每花序的花蕾数，表明VvCKX 5可能是葡萄中每花序花数的负调控因子之一。类似地，葡萄分枝3的姐妹（VvSRA），其编码海藻糖6-磷酸酶，催化海藻糖-6-磷酸转化为海藻糖，上调AtCKX 7在拟南芥植物中的表达，导致每个拟南芥花序的花蕾数量减少。VvCKX 5基因表达上调葡萄藤培养细胞和幼年葡萄花序用海藻糖处理。最后，使用微量注射器将海藻糖注射到接近萌芽的肿胀芽中，每串浆果的数量减少了一半。拟南芥植物中VvCKX 5过表达对主花序的次生花序的数量没有影响，同样海藻糖也没有影响葡萄藤花序上的花梗分枝，这表明VvCKX 5以及VvSRA介导的海藻糖代谢调节花的形成，但不调节花序分枝。这些发现可能提供新的信息之间的串扰VvSRA介导的海藻糖代谢和VvCKX介导的细胞分裂素降解确定每串浆果的数量。此外，这项研究预计将有助于开发创新的栽培技术，放松紧束。
In grapes, the number of flowers per inflorescence determines the compactness of grape bunches. Grape cultivars with tight bunches and thin-skinned berries easily undergo berry splitting, especially in growing areas with heavy rainfall during the grapevine growing season, such as Japan. We report herein that grape cytokinin oxidase/dehydrogenase 5 (VvCKX5) determines the number of berries per inflorescence in grapes. The number of berries per bunch was inversely proportional to the VvCKX5 expression level in juvenile inflorescences among the cultivars tested. VvCKX5 overexpression drastically decreased the number of flower buds per inflorescence in Arabidopsis plants, suggesting that VvCKX5 might be one of the negative regulators of the number of flowers per inflorescence in grapes. Similarly, the overexpression of grape sister of ramose 3 (VvSRA), which encodes trehalose 6-phosphate phosphatase that catalyzes the conversion of trehalose-6-phosphate into trehalose, upregulated AtCKX7 expression in Arabidopsis plants, leading to a decrease in the number of flower buds per Arabidopsis inflorescence. VvCKX5 gene expression was upregulated in grapevine cultured cells and juvenile grape inflorescences treated with trehalose. Finally, injecting trehalose into swelling buds nearing bud break using a microsyringe decreased the number of berries per bunch by half. VvCKX5 overexpression in Arabidopsis plants had no effect on the number of secondary inflorescences from the main inflorescence, and similarly trehalose did not affect pedicel branching on grapevine inflorescences, suggesting that VvCKX5, as well as VvSRA-mediated trehalose metabolism, regulates flower formation but not inflorescence branching. These findings may provide new information on the crosstalk between VvSRA-mediated trehalose metabolism and VvCKX-mediated cytokinin degradation for determining the number of berries per bunch. Furthermore, this study is expected to contribute to the development of innovative cultivation techniques for loosening tight bunches.
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影响因子：
3.5
作者：
Liu P;Zhang C;Ma JQ;Zhang LY;Yang B;Tang XY;Huang L;Zhou XT;Lu K;Li JN
通讯作者：
Li JN
影响因子：
3.5
作者：
Ali, Q.;Ashraf, M.
通讯作者：
Ashraf, M.
影响因子：
3
作者：
Ishiai, Shiho;Nakajima, Yuko;Suzuki, Shunji
通讯作者：
Suzuki, Shunji
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
4.6
作者：
Gallavotti, Andrea;Long, Jeff A.;Schmidt, Robert J.
通讯作者：
Schmidt, Robert J.