Genome-Wide Identification and Expression Profiling of Cytokinin Oxidase/Dehydrogenase (CKX) Genes Reveal Likely Roles in Pod Development and Stress Responses in Oilseed Rape (Brassica napus L.).
Genome-Wide Identification and Expression Profiling of Cytokinin Oxidase/Dehydrogenase (CKX) Genes Reveal Likely Roles in Pod Development and Stress Responses in Oilseed Rape (Brassica napus L.).
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作者：
Liu P;Zhang C;Ma JQ;Zhang LY;Yang B;Tang XY;Huang L;Zhou XT;Lu K;Li JN
细胞分裂素氧化酶/脱氢酶(CKXs)在细胞分裂素的不可逆降解过程中起着关键作用，从而调节植物的生长发育。甘蓝型油菜是世界上种植面积最大的油料作物之一。随着甘蓝型油菜全基因组测序的完成，对BnCKX基因家族进行全基因组鉴定和表达分析在技术上已经成为可能。在这项研究中，我们鉴定了23个BnCKX基因，并分析了它们的系统发育关系、基因结构、保守基序、蛋白质亚细胞定位等特性。我们还利用定量逆转录聚合酶链式反应(qRT-PCR)分析了23个BnCKX基因在甘蓝型油菜品种中双11(‘ZS11’)中的表达，揭示了它们的差异表达模式。根据RNA测序和qRT-PCR的结果，我们选择了4个BnCKX基因，并比较了它们在超长和短角果栽培品种中的表达。BnCKX5-1、5-2、6-1和7-1的表达水平在两个品系之间差异显著，并在角果发育过程中发生变化，表明它们可能在角果长度的决定和角果发育中发挥作用。最后，我们研究了合成的细胞分裂素6-苄基氨基嘌呤(6-BA)和生长素吲哚-3-乙酸(IAA)处理对所选的4个BnCKX基因表达的影响。我们的结果表明，调控BnCKX的表达是提高植物收获指数和抗逆性的一条很有前途的途径。
Cytokinin oxidase/dehydrogenases (CKXs) play a critical role in the irreversible degradation of cytokinins, thereby regulating plant growth and development. Brassica napus is one of the most widely cultivated oilseed crops worldwide. With the completion of whole-genome sequencing of B. napus, genome-wide identification and expression analysis of the BnCKX gene family has become technically feasible. In this study, we identified 23 BnCKX genes and analyzed their phylogenetic relationships, gene structures, conserved motifs, protein subcellular localizations, and other properties. We also analyzed the expression of the 23 BnCKX genes in the B. napus cultivar Zhong Shuang 11 (‘ZS11’) by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), revealing their diverse expression patterns. We selected four BnCKX genes based on the results of RNA-sequencing and qRT-PCR and compared their expression in cultivated varieties with extremely long versus short siliques. The expression levels of BnCKX5-1, 5-2, 6-1, and 7-1 significantly differed between the two lines and changed during pod development, suggesting they might play roles in determining silique length and in pod development. Finally, we investigated the effects of treatment with the synthetic cytokinin 6-benzylaminopurine (6-BA) and the auxin indole-3-acetic acid (IAA) on the expression of the four selected BnCKX genes. Our results suggest that regulating BnCKX expression is a promising way to enhance the harvest index and stress resistance in plants.
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影响因子：
4.8
作者：
Gaudinová, A;Dobrev, PI;Motyka, V
通讯作者：
Motyka, V
影响因子：
7.4
作者：
Bilyeu, KD;Cole, JL;Morris, RO
通讯作者：
Morris, RO
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
5.8
作者：
Huelsenbeck, JP;Ronquist, F
通讯作者：
Ronquist, F