Genome-Wide Identification and Characterization of the Cyclophilin Gene Family in the Nematophagous Fungus Purpureocillium lilacinum
Genome-Wide Identification and Characterization of the Cyclophilin Gene Family in the Nematophagous Fungus Purpureocillium lilacinum
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食线虫紫紫霉亲环蛋白基因家族的全基因组鉴定和表征
DOI：
10.3390/ijms20122978
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发表时间：
2019-06
影响因子：
5.6
通讯作者：
Yannong Xiao
中科院分区：
文献类型：
--
作者：
Chenmi Mo;Chong Xie;Gaofeng Wang;Juan Liu;Qiuyan Hao;Xueqiong Xiao;Yannong Xiao
淡紫紫青霉是一种广泛应用于植物寄生线虫防治的生物防治剂。全基因组分析促进了功能基因的鉴定及其分子机制的探索。亲环蛋白（Cyclophilin，CLD）基因家族属于亲免蛋白超家族，具有一个保守的亲环蛋白样结构域（cyclophilin-like domain，CLD）。CYP广泛存在于原核生物和真核生物中，可分为单结构域和多结构域蛋白。本研究从淡紫青霉36-1的基因组中鉴定出10个具有CLD的基因，命名为PlCYP 1-P10。预测这10种PlCYP在淡紫青霉中具有不同的细胞定位。系统发育分析和基因结构分析表明，CYP在子囊菌亚门和子囊菌亚门真菌中存在进化分化，但在子囊菌亚门真菌中具有保守性。基序和基因结构分布进一步支持了系统发育分析的结果。Plastin基因在淡紫拟单胞菌不同发育阶段和寄生于南方根结线虫卵上的阶段均有特异性表达。此外，10个PlCYP基因在响应非生物胁迫时表现出不同的表达丰度，其中PlCYP 4在高温（35 °C）下高表达，而PlCYP 6在5 mM H2 O2胁迫下上调。此外，PlCYP 4和PlCYP 6在大肠杆菌中的异源表达增强了细胞对高温和H2 O2的耐受性。综上所述，本研究揭示了PlCYP在子囊菌亚门真菌毒力和胁迫反应中的潜在功能，为进一步研究PlCYP家族在子囊菌亚门真菌中的功能提供了框架。
Purpureocillium lilacinum has been widely used as a commercial biocontrol agent for the control of plant parasitic nematodes. Whole genome analysis promotes the identification of functional genes and the exploration of their molecular mechanisms. The Cyclophilin (CYP) gene family belongs to the immunophillin superfamily, and has a conserved cyclophilin-like domain (CLD). CYPs are widely identified in prokaryotes and eukaryotes, and can be divided into single- and multi-domain proteins. In the present study, 10 CYP genes possessing the CLD, named PlCYP1–P10, were identified from the genome of P. lilacinum strain 36-1. Those 10 PlCYPs were predicted to have different cellular localizations in P. lilacinum. Phylogenetic and gene structure analysis revealed the evolutionary differentiation of CYPs between Ascomycotina and Saccharomycotina fungi, but conservation within the Ascomycotina fungi. Motif and gene structure distributions further support the result of phylogenetic analysis. Each PlCYP gene had a specific expression pattern in different development stages of P. lilacinum and its parasitism stage on eggs of Meloidogyne incognita. In addition, the 10 PlCYP genes exhibited different expression abundances in response to abiotic stresses, among which PlCYP4 was highly expressed at a high temperature (35 °C), while PlCYP6 was up-regulated under 5 mM of H2O2 stress. Furthermore, the heterologous expression of PlCYP4 and PlCYP6 in Escherichia coli enhanced the cellular tolerance against a high temperature and H2O2. In summary, our study indicates the potential functions of PlCYPs in virulence and the stress response, and also provides a frame for further analysis of the CYP gene family in Ascomycotina fungi.
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