Pemphigus vulgaris and pemphigus foliaceus sera show an inversely graded binding pattern to extracellular regions of desmosomes in different layers of human epidermis.
Pemphigus vulgaris and pemphigus foliaceus sera show an inversely graded binding pattern to extracellular regions of desmosomes in different layers of human epidermis.
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寻常型天疱疮和落叶型天疱疮血清显示出与人表皮不同层中桥粒细胞外区域的反梯度结合模式。
DOI：
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发表时间：
1995
影响因子：
6.5
通讯作者：
T. Nishikawa
中科院分区：
文献类型：
--
作者：
Hiroshi Shimizu;T. Masunaga;A. Ishiko;A. Kikuchi;Takashi Hashimoto;T. Nishikawa
利用3例寻常型天疱疮和3例叶型天疱疮患者的血清标本，分析了寻常型天疱疮(PV)和叶型天疱疮(PF)抗原在人表皮中的结合部位。激光共聚焦扫描显微镜、超薄冰冻切片免疫荧光检查和免疫过氧化物酶电子显微镜显示沿表皮细胞表面有不连续的圆点。超薄冰冻切片免疫金电镜示PV和PF自身抗体仅与桥粒特异结合。包埋后采用冷冻固定和冷冻置换的免疫金电子显微镜能够检查整个表皮的深度，并通过计数金颗粒来定量PV和PF自身抗体的结合。PV和PF自身抗体均与表皮中的所有桥粒结合，但不与非桥粒角质形成细胞表面结合。大多数自身抗体结合发生在胞外区(PV，62%；PF，69%)。关于标记单个桥粒的金颗粒数量的双向方差分析的统计分析证实，天疱疮亚型(PV和Pf)与不同的表皮细胞层之间存在显著的相互作用(p<；0.044)。结果表明，PV血清与单个桥粒结合的金粒数在下表皮显著多于上表皮，且PF血清的金粒数呈倒数分布。这种反向分级的结合模式表明桥粒组成的异质性，这可能解释了PV和PF棘层松解程度的差异。
We analyzed the location of binding sites for pemphigus vulgaris (PV) antigen and pemphigus foliaceus (PF) antigen in the human epidermis using serum samples obtained from three patients with PV and three patients with PF. Confocal laser scanning microscopy, immunofluorescent examination of ultrathin cryosections, and immunoperoxidase electron microscopy demonstrated discontinuous dots along the epidermal cell surfaces. Immunogold electron microscopy of ultrathin cryosections showed specific binding of PV and PF autoantibodies only to desmosomes. Post-embedding immunogold electron microscopy using cryofixation and cryosubstitution enabled the whole depth of the epidermis to be examined and the binding of PV and PF autoantibodies to be quantitated by counting gold particles. Both PV and PF autoantibodies bound to all desmosomes in the epidermis, but not to the surface of the non-desmosomal keratinocytes. The majority of auto-antibody binding occurred in the extracellular domain (PV, 62%; PF, 69%). The statistical analysis of two-way analysis of variance regarding the number of gold particles labeling a single desmosome confirmed a significant interaction between subtypes of pemphigus (PV and PF) and the different epidermal cell layers (p < 0.044). The results indicate that the number of gold particles bound to individual desmosomes with PV sera was significantly higher in the lower epidermis than in the upper epidermis, and that of PF sera showed reciprocal pattern. This inversely graded binding pattern suggests heterogeneity of the composition of the desmosomes, which may explain the differences in level of acantholysis between PV and PF.
DOI：
10.1111/1523-1747.ep12474454
发表时间：
1991
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Calvanico,NJ;Martins,CR;Diaz,LA
通讯作者：
Diaz,LA
抗桥粒的人类自身抗体：天疱疮的可能致病因素。
DOI：
10.1073/pnas.81.9.2781
发表时间：
1984
影响因子：
11.1
作者：
Jones,JC;Arnn,J;Staehelin,LA;Goldman,RD
通讯作者：
Goldman,RD