Intensity response function of the photopic negative response (PhNR): effect of age and test-retest reliability.
Intensity response function of the photopic negative response (PhNR): effect of age and test-retest reliability.
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DOI：
10.1007/s10633-017-9591-0
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Viswanathan S
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文献类型：
--
作者：
Joshi NR;Ly E;Viswanathan S
评估正常健康人全视野明视负反应（PhNR）强度反应函数的年龄效应和重测信度。从45名受试者的一只眼睛记录全视野视网膜电图（ERG），其中39名受试者在两天内使用Diagnosys Espion系统（Lowell，MA，USA）进行测试。视觉刺激由在恒定的7 cd/m2蓝色背景上递送的范围为0.000625至6.4 phot cd s/m2的短暂（<5 ms）红色闪光组成。在其谷值处测量PhNR振幅，从基线（BT）和之前的b波峰值（PT）开始，如果a波不存在，则在其峰值处测量b波振幅，从之前的a波谷值或基线开始。用广义Naka-Rushton函数拟合所有三种ERG测量的强度响应数据，以导出饱和振幅（Vmax）、半饱和常数（K）和斜率（n）参数。年龄对拟合参数的影响采用线性回归进行评估，重测信度采用Wilcoxon符号秩检验和Bland-Altman分析进行评估。应用霍尔姆校正来解释多重比较。BT的Vmax显著小于PT和b波，PT和b波的Vmax无显著性差异。BT的斜率参数n最小，b波的斜率参数n最大，所有三种测量的斜率之间的差异具有统计学显著性。在不同测量的K平均值中观察到的微小差异未达到统计学显著性。Wilcoxon符号秩检验表明两次检查访视之间三种ERG测量的任何Naka-Rushton参数均无显著差异，Bland-Altman图表明不同拟合参数的试验和复检测量值之间的平均差异接近于零，并且在所有三种ERG测量的相应参数的试验和复检值平均值的6%范围内，表明偏差很小虽然每个拟合参数的可靠性系数（COR，定义为测试和重新测试差异的标准差的1.96倍）在三个ERG测量值之间或多或少相当，但对于每个拟合参数，BT的%COR（COR标准化为平均测试和重新测试测量值）通常大于PT和b波。多重比较校正时，Naka-Rushton拟合参数未显示任何ERG指标随年龄的统计学显著变化。然而，在校正多重比较之前，BT的Vmax与年龄相关性较弱，当测量值表示为同一受试者b波Vmax的比值时，年龄对该参数的影响具有更大的显著性。PhNR的BT幅度测量的Vmax在最好的情况下与年龄弱相关。Naka-Rushton拟合PhNR或b波强度反应数据的其他参数均未显示出随年龄的任何系统性变化。PhNR BT振幅测量的拟合参数的重测信度似乎低于PhNR PT和b波振幅测量。
To assess the effect of age and test-retest reliability of the intensity response function of the full field Photopic Negative Response (PhNR) in normal healthy human subjects. Full field electroretinograms (ERGs) were recorded from one eye of 45 subjects and 39 of these subjects were tested on two separate days with a Diagnosys Espion System (Lowell, MA, USA). The visual stimuli consisted of brief (<5ms) red flashes ranging from 0.000625 to 6.4 phot cd s/m2, delivered on a constant 7 cd/m2 blue background. PhNR amplitudes were measured at its trough from baseline (BT) and from the preceding b-wave peak (PT) and b-wave amplitude was measured at its peak from the preceding a-wave trough or baseline if the a-wave was not present. The intensity response data of all three ERG measures was fitted with a generalized Naka-Rushton function to derive the saturated amplitude (Vmax), semisaturation constant (K) and slope (n) parameters. Effect of age on the fit parameters was assessed with linear regression and test-retest reliability was assessed with the Wilcoxon Signed Rank test and Bland-Altman analysis. Holm’s correction was applied to account for multiple comparisons. Vmax of BT was significantly smaller than that of PT and b-wave and the Vmax of PT and b-wave were not significantly different from each other. The slope parameter n was smallest for BT and largest for b-wave and the difference between the slopes of all three measures were statistically significant. Small differences observed in the mean values of K for the different measures did not reach statistical significance. The Wilcoxon signed rank test indicated no significant differences between the two test visits for any of the Naka-Rushton parameters for the three ERG measures and the Bland-Altman plots indicated that the mean difference between test and retest measurements of the different fit parameters were close to zero and within 6% of the average of the test and retest values of the respective parameters for all three ERG measurements, indicating minimal bias. While the Coefficient of Reliability (COR, defined as 1.96 times the standard deviation of the test and retest difference) of each fit parameter was more or less comparable across the three ERG measurements, the %COR (COR normalized to the mean test and retest measures) was generally larger for BT compared to both PT and b-wave for each fit parameter. The Naka-Rushton fit parameters did not show statistically significant changes with age for any of the ERG measures when corrections were applied for multiple comparisons. However, the Vmax of BT demonstrated a weak correlation with age prior to correction for multiple comparisons and the effect of age on this parameter showed greater significance when the measure was expressed as a ratio of the Vmax of the b-wave from the same subject. The Vmax of the BT amplitude measure of PhNR at the best was weakly correlated with age. None of the other parameters of the Naka-Rushton fit to the intensity response data of either the PhNR or the b-wave showed any systematic changes with age. The test-retest reliability of the fit parameters for PhNR BT amplitude measurements appear to be lower than those of the PhNR PT and b-wave amplitude measurements.
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DOI：
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发表时间：
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期刊：
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