Metagenomic comparisons reveal a highly diverse and unique viral community in a seasonally fluctuating hypersaline microbial mat.
Metagenomic comparisons reveal a highly diverse and unique viral community in a seasonally fluctuating hypersaline microbial mat.
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DOI：
10.1099/mgen.0.001063
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发表时间：
2023-07
影响因子：
3.9
通讯作者：
Souza, Valeria
中科院分区：
文献类型：
--
作者：
Cisneros-Martinez, Alejandro Miguel;Eguiarte, Luis E.;Souza, Valeria
2016年春天，在Cuatro Cienegas盆地（CCB）发现了一个浅的高盐池塘（50×25米）。这个池塘，被称为古地中海圆顶（AD），因为它的弹性微生物垫，形成圆顶形结构，由于生产的还原性气体，让人想起古地中海时代，如甲烷和硫化氢，港口高度多样化的微生物群落，富含嗜盐和产甲烷古菌。AD是一个季节性波动的高盐网站，盐度范围从低高盐（5.3%）在雨季高盐（饱和）在旱季。为了表征病毒群落，并测试它是否类似于其他高盐地区（其多样性受盐度的影响），或者它是否类似于其他CCB地区（与其共享共同的地质历史），我们在4年内从不同季节和深度生成了12个宏基因组，并将其与来自不同环境的35个宏基因组进行了比较。检测到盐古病毒，但从未占优势（平均占总病毒种类的15. 37%），病毒群落结构和多样性不受环境波动的影响。 事实上，与高盐地区的其他病毒群落不同，无论季节如何，AD都比其他环境更加多样化。β-多样性分析表明，AD与其他CCB位点密切相关，尽管它有一个独特的病毒群落，形成了自己的簇。两个表面样品的相似性30和50厘米深的样品，以及观察到的增加，在更深的深度的多样性，支持的假设，CCB的多样性已经演变为一个长期的环境稳定的深层含水层，功能作为一个“种子银行”的伟大的微生物多样性，被运送到表面的零星地下水涌上事件的结果。
In spring 2016, a shallow hypersaline pond (50×25 m) was found in the Cuatro Cienegas Basin (CCB). This pond, known as Archaean Domes (AD) because of its elastic microbial mats that form dome-shaped structures due to the production of reducing gases reminiscent of the Archaean eon, such as methane and hydrogen sulfide, harbour a highly diverse microbial community, rich in halophilic and methanogenic archaea. AD is a seasonally fluctuating hypersaline site, with salinity ranging from low hypersaline (5.3%) during the wet season to high hypersaline (saturation) during the dry season. To characterize the viral community and to test whether it resembles those of other hypersaline sites (whose diversity is conditioned by salinity), or if it is similar to other CCB sites (with which it shares a common geological history), we generated 12 metagenomes from different seasons and depths over a 4 year period and compared them to 35 metagenomes from varied environments. Haloarchaeaviruses were detected, but were never dominant (average of 15.37 % of the total viral species), and the viral community structure and diversity were not affected by environmental fluctuations. In fact, unlike other viral communities at hypersaline sites, AD remained more diverse than other environments regardless of season. β-Diversity analyses show that AD is closely related to other CCB sites, although it has a unique viral community that forms a cluster of its own. The similarity of two surface samples to the 30 and 50 cm depth samples, as well as the observed increase in diversity at greater depths, supports the hypothesis that the diversity of CCB has evolved as a result of a long time environmental stability of a deep aquifer that functions as a ‘seed bank’ of great microbial diversity that is transported to the surface by sporadic groundwater upwelling events.
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影响因子：
3.7
作者：
Boros E;Kolpakova M
通讯作者：
Kolpakova M
影响因子：
3.7
作者：
Garcia-Heredia I;Martin-Cuadrado AB;Mojica FJ;Santos F;Mira A;Antón J;Rodriguez-Valera F
通讯作者：
Rodriguez-Valera F
影响因子：
1.4
作者：
GuixaBoixareu, N;CalderonPaz, JI;PedrosAlio, C
通讯作者：
PedrosAlio, C
影响因子：
2.6
作者：
Ash, Kurt T.;Drake, Kristina M.;Ely, Bert
通讯作者：
Ely, Bert
影响因子：
5.2
作者：
Garcia-Ulloa, Manuel;Souza, Valeria;Esquivel-Hernandez, Diego A.;Sanchez-Perez, Jazmin;Espinosa-Asuar, Laura;Viladomat, Mariette;Marroquin-Rodriguez, Montserrat;Navarro-Miranda, Marisol;Ruiz-Padilla, Jair;Monroy-Guzman, Camila;Madrigal-Trejo, David;Rosas-Barrera, Manuel;Vazquez-Rosas-Landa, Mirna;Eguiarte, Luis E.
通讯作者：
Eguiarte, Luis E.