FgBud3, a Rho4-Interacting Guanine Nucleotide Exchange Factor, Is Involved in Polarity Growth, Cell Division and Pathogenicity of Fusarium graminearum.
FgBud3, a Rho4-Interacting Guanine Nucleotide Exchange Factor, Is Involved in Polarity Growth, Cell Division and Pathogenicity of Fusarium graminearum.
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FgBud3 是一种与 Rho4 相互作用的鸟嘌呤核苷酸交换因子，参与禾谷镰刀菌的极性生长、细胞分裂和致病性
DOI：
10.3389/fmicb.2018.01209
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Zhang C;Luo Z;He D;Su L;Yin H;Wang G;Liu H;Rensing C;Wang Z
Rho GTP酶是与禾谷镰刀菌的发育进程和发病机制相关的信号大分子。一般来说，Rho GTP酶的活性受Rho GTP酶鸟嘌呤核苷酸交换因子(RhoGEF)的调节。在本研究中，我们从禾谷镰刀菌的数据库中鉴定了一个可能的Rhogef编码基因(FgBUD3)，并进一步利用功能遗传学的方法产生了针对FgBUD3的基因缺失突变体。表型分析结果表明，FgBUD3的缺失导致本研究期间产生的FgBUD3突变体的生长严重减慢。我们还观察到，在ΔFgbuD3株中，FgBUD3基因的缺失完全取消了有性生殖，并引发了几乎没有隔膜的异常无性孢子的产生。进一步的侵染分析结果表明，FgBUD3缺陷突变体在小麦上失去了致病性，因此，FgBud3在小麦赤霉病的致病中起着重要作用。此外，酵母双杂交实验结果表明，FgBud3与FgRho4的相互作用强于FgRho2、FgRho3和FgCdc42。此外，我们还发现FgBud3与GTP结合形式和GDP结合形式的FgRho4相互作用。根据这些结果，我们随后得出结论，与Rho4相互作用的环境基金蛋白FgBud3对禾谷镰刀菌的营养生长、无性和有性发育、细胞分裂和致病性具有至关重要的促进作用。
Rho GTPases are signaling macromolecules that are associated with developmental progression and pathogenesis of Fusarium graminearum. Generally, enzymatic activities of Rho GTPases are regulated by Rho GTPase guanine nucleotide exchange factors (RhoGEFs). In this study, we identified a putative RhoGEF encoding gene (FgBUD3) in F. graminearum database and proceeded further by using a functional genetic approach to generate FgBUD3 targeted gene deletion mutant. Phenotypic analysis results showed that the deletion of FgBUD3 caused severe reduction in growth of FgBUD3 mutant generated during this study. We also observed that the deletion of FgBUD3 completely abolished sexual reproduction and triggered the production of abnormal asexual spores with nearly no septum in ΔFgbud3 strain. Further results obtained from infection assays conducted during this research revealed that the FgBUD3 defective mutant lost its pathogenicity on wheat and hence, suggests FgBud3 plays an essential role in the pathogenicity of F. graminearum. Additional, results derived from yeast two-hybrid assays revealed that FgBud3 strongly interacted with FgRho4 compared to the interaction with FgRho2, FgRho3, and FgCdc42. Moreover, we found that FgBud3 interacted with both GTP-bound and GDP-bound form of FgRho4. From these results, we subsequently concluded that, the Rho4-interacting GEF protein FgBud3 crucially promotes vegetative growth, asexual and sexual development, cell division and pathogenicity in F. graminearum.
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