Evidence for lateral gene transfer (LGT) in the evolution of eubacteria-derived small GTPases in plant organelles.
Evidence for lateral gene transfer (LGT) in the evolution of eubacteria-derived small GTPases in plant organelles.
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在植物细胞器中衍生的小细菌衍生的小GTP酶进化中侧基因转移（LGT）的证据。
DOI：
10.3389/fpls.2014.00678
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发表时间：
2014
影响因子：
5.6
通讯作者：
Shiina T
中科院分区：
文献类型：
--
作者：
Suwastika IN;Denawa M;Yomogihara S;Im CH;Bang WY;Ohniwa RL;Bahk JD;Takeyasu K;Shiina T
自由生活细菌的基因组通过横向基因转移（LGT）频繁交换基因，这在细菌进化中起着重要作用。LGT在从非蓝藻细菌到“初级”藻类和陆地植物谱系的基因获取中也发挥了重要作用。小gtp酶广泛分布于原核生物和真核生物中。在这项研究中，我们推断了植物细胞器靶向小gtpase的进化史。拟南芥在OBG-HflX-like和TrmE-Era-EngA-YihA-Septin-like GTPase超家族（合称为Era-like GTPases）的7个亚家族中至少含有一个同源物。对拟南芥中所有era样gtpase的亚细胞定位分析表明，30个真细菌相关的gtpase全部定位于叶绿体和/或线粒体，而古细菌相关的DRG和NOG1分别定位于细胞质和细胞核，表明叶绿体和线粒体定位的gtpase分别来自于蓝藻和α-变形菌的祖先，通过内共生基因转移（EGT）。然而，系统发育分析表明，植物细胞器GTPase的进化相当复杂。在与真细菌相关的GTPase中，来自蓝藻菌和α-变形菌的GTPase分别只有4个定位于叶绿体（包括1个双靶向GTPase）和2个定位于线粒体。另外三种叶绿体靶向gtpase与α-变形菌蛋白相关，而与蓝藻gtpase无关。此外，我们发现另外四个gtpase既不属于蓝藻菌，也不属于α-变形菌。相反，这些gtpase与其他真细菌的分支密切相关，如拟杆菌（Era1， EngB-1和EngB-2）和绿色非硫细菌（HflX）。因此，该研究提供了新的证据，证明LGT在植物细胞器靶向era样gtpase的进化中发挥了重要作用。
The genomes of free-living bacteria frequently exchange genes via lateral gene transfer (LGT), which has played a major role in bacterial evolution. LGT also played a significant role in the acquisition of genes from non-cyanobacterial bacteria to the lineage of “primary” algae and land plants. Small GTPases are widely distributed among prokaryotes and eukaryotes. In this study, we inferred the evolutionary history of organelle-targeted small GTPases in plants. Arabidopsis thaliana contains at least one ortholog in seven subfamilies of OBG-HflX-like and TrmE-Era-EngA-YihA-Septin-like GTPase superfamilies (together referred to as Era-like GTPases). Subcellular localization analysis of all Era-like GTPases in Arabidopsis revealed that all 30 eubacteria-related GTPases are localized to chloroplasts and/or mitochondria, whereas archaea-related DRG and NOG1 are localized to the cytoplasm and nucleus, respectively, suggesting that chloroplast- and mitochondrion-localized GTPases are derived from the ancestral cyanobacterium and α-proteobacterium, respectively, through endosymbiotic gene transfer (EGT). However, phylogenetic analyses revealed that plant organelle GTPase evolution is rather complex. Among the eubacterium-related GTPases, only four localized to chloroplasts (including one dual targeting GTPase) and two localized to mitochondria were derived from cyanobacteria and α-proteobacteria, respectively. Three other chloroplast-targeted GTPases were related to α-proteobacterial proteins, rather than to cyanobacterial GTPases. Furthermore, we found that four other GTPases showed neither cyanobacterial nor α-proteobacterial affiliation. Instead, these GTPases were closely related to clades from other eubacteria, such as Bacteroides (Era1, EngB-1, and EngB-2) and green non-sulfur bacteria (HflX). This study thus provides novel evidence that LGT significantly contributed to the evolution of organelle-targeted Era-like GTPases in plants.
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影响因子：
7.4
作者：
BAUMGARTNER, BJ;RAPP, JC;MULLET, JE
通讯作者：
MULLET, JE
影响因子：
2.1
作者：
Hirano, Yasuhiro;Ohniwa, Ryosuke L.;Takeyasu, Kunio
通讯作者：
Takeyasu, Kunio
影响因子：
3.5
作者：
Grauvogel, Carina;Petersen, Joern
通讯作者：
Petersen, Joern
影响因子：
4.8
作者：
Jensen, BC;Wang, Q;Parsons, M
通讯作者：
Parsons, M
影响因子：
13.8
作者：
Jeanmougin, F;Thompson, JD;Gibson, TJ
通讯作者：
Gibson, TJ