Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions.
Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions.
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人类精液中的外泌体具有具有潜在调节功能的小型非编码RNA的独特曲目。
DOI：
10.1093/nar/gku347
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发表时间：
2014-06
影响因子：
14.9
通讯作者：
Hladik F
中科院分区：
文献类型：
--
作者：
Vojtech L;Woo S;Hughes S;Levy C;Ballweber L;Sauteraud RP;Strobl J;Westerberg K;Gottardo R;Tewari M;Hladik F
精液中含有相对不明确的调节成分，这些成分可能有助于受精，但也可能干扰对感染的防御。每一次射精都含有数万亿个外泌体，膜封闭的亚细胞微泡，对生殖器粘膜中重要的细胞具有免疫抑制作用。外泌体通常被认为可能通过转移小RNA分子来介导细胞间通讯。我们发现，精液外泌体（SE）制剂含有大量的RNA从20至100个核苷酸（nt）的长度。我们分别从6个精液供体中测序了20-40和40-100 nt的SE RNA片段。我们发现了各种类型的小的非编码RNA，包括microRNA（20-40 nt部分中RNA的21.7%）以及存在于两个部分中的丰富的Y RNA和tRNA。特异性RNA在所有供体中一致存在。例如，在SE中，10种（已知的12600种）microRNA占成熟microRNA的40%以上。此外，tRNA片段在长度为18-19或30-34 nt的5 '端高度富集;这样的tRNA片段抑制翻译。因此，SE可能通过转移小RNA分子向受体粘膜传递调节信号。
Semen contains relatively ill-defined regulatory components that likely aid fertilization, but which could also interfere with defense against infection. Each ejaculate contains trillions of exosomes, membrane-enclosed subcellular microvesicles, which have immunosuppressive effects on cells important in the genital mucosa. Exosomes in general are believed to mediate inter-cellular communication, possibly by transferring small RNA molecules. We found that seminal exosome (SE) preparations contain a substantial amount of RNA from 20 to 100 nucleotides (nts) in length. We sequenced 20–40 and 40–100 nt fractions of SE RNA separately from six semen donors. We found various classes of small non-coding RNA, including microRNA (21.7% of the RNA in the 20–40 nt fraction) as well as abundant Y RNAs and tRNAs present in both fractions. Specific RNAs were consistently present in all donors. For example, 10 (of ∼2600 known) microRNAs constituted over 40% of mature microRNA in SE. Additionally, tRNA fragments were strongly enriched for 5’-ends of 18–19 or 30–34 nts in length; such tRNA fragments repress translation. Thus, SE could potentially deliver regulatory signals to the recipient mucosa via transfer of small RNA molecules.
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影响因子：
21.3
作者：
Hergenreider, Eduard;Heydt, Susanne;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
影响因子：
64.5
作者：
Chen X;Taylor DW;Fowler CC;Galan JE;Wang HW;Wolin SL
通讯作者：
Wolin SL
影响因子：
14.9
作者：
Flicek P;Amode MR;Barrell D;Beal K;Billis K;Brent S;Carvalho-Silva D;Clapham P;Coates G;Fitzgerald S;Gil L;Girón CG;Gordon L;Hourlier T;Hunt S;Johnson N;Juettemann T;Kähäri AK;Keenan S;Kulesha E;Martin FJ;Maurel T;McLaren WM;Murphy DN;Nag R;Overduin B;Pignatelli M;Pritchard B;Pritchard E;Riat HS;Ruffier M;Sheppard D;Taylor K;Thormann A;Trevanion SJ;Vullo A;Wilder SP;Wilson M;Zadissa A;Aken BL;Birney E;Cunningham F;Harrow J;Herrero J;Hubbard TJ;Kinsella R;Muffato M;Parker A;Spudich G;Yates A;Zerbino DR;Searle SM
通讯作者：
Searle SM
DOI：
10.1099/vir.0.044255-0
发表时间：
2012-11
期刊：
The Journal of general virology
影响因子：
--
作者：
Bakre A;Mitchell P;Coleman JK;Jones LP;Saavedra G;Teng M;Tompkins SM;Tripp RA
通讯作者：
Tripp RA
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S