Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress.
Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress.
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DOI：
10.1186/gb-2006-7-2-r9
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Werner-Washburne M
中科院分区：
文献类型：
--
作者：
Aragon AD;Quiñones GA;Thomas EV;Roy S;Werner-Washburne M
描述了由于响应于应激从酵母细胞释放抗提取mRNA而导致的快速转录物增加。随着碳源耗尽，酿酒酵母细胞表现出降低的代谢活性，培养物进入稳定期。我们询问静止期培养物中的细胞是否在转录丰度水平上对额外的应激作出反应。微阵列被用来量化响应于应激的来自稳定期培养物的细胞中的转录物丰度的变化。超过800种mRNA在氧化应激后一分钟大量增加。这些mRNA中有相当数量编码参与应激反应的蛋白质。我们测试了mRNA的增加是否是由于新的转录、信息的快速多聚腺苷酸化（这不会被微阵列检测到）或细胞中存在的成熟mRNA的潜在释放，但在RNA分离过程中对提取有抵抗力。在RNA聚合酶II突变体，rpb 1 -1，对氧化应激的反应的检查表明，不需要新的转录。定量RT-PCR分析的一个子集，这些成绩单进一步表明，存在于分离的总RNA从稳定期培养物的成绩单聚腺苷酸化。相比之下，超过2,000个转录物在蛋白酶处理来自稳定期但不是指数生长培养物的无细胞裂解物后增加。不同子集的转录本释放氧化应激和温度升高，这表明mRNA的释放是应激特异性的。稳定期培养物中的细胞含有大量蛋白酶不稳定、快速释放形式的抗提取mRNA。转录本的释放似乎是压力特异性的。我们假设这些转录本与P体有关。
A rapid transcript increase due to the release of extraction-resistant mRNAs from yeast cells in response to stress is described. As carbon sources are exhausted, Saccharomyces cerevisiae cells exhibit reduced metabolic activity and cultures enter the stationary phase. We asked whether cells in stationary phase cultures respond to additional stress at the level of transcript abundance. Microarrays were used to quantify changes in transcript abundance in cells from stationary phase cultures in response to stress. More than 800 mRNAs increased in abundance by one minute after oxidative stress. A significant number of these mRNAs encode proteins involved in stress responses. We tested whether mRNA increases were due to new transcription, rapid poly-adenylation of message (which would not be detected by microarrays), or potential release of mature mRNA present in the cell but resistant to extraction during RNA isolation. Examination of the response to oxidative stress in an RNA polymerase II mutant, rpb1-1, suggested that new transcription was not required. Quantitative RT-PCR analysis of a subset of these transcripts further suggested that the transcripts present in isolated total RNA from stationary phase cultures were polyadenylated. In contrast, over 2,000 transcripts increased after protease treatment of cell-free lysates from stationary phase but not exponentially growing cultures. Different subsets of transcripts were released by oxidative stress and temperature upshift, suggesting that mRNA release is stress-specific. Cells in stationary phase cultures contain a large number of extraction-resistant mRNAs in a protease-labile, rapidly releasable form. The transcript release appears to be stress-specific. We hypothesize that these transcripts are associated with P-bodies.
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影响因子：
3.6
作者：
Grant, CM;Collinson, LP;Dawes, IW
通讯作者：
Dawes, IW
影响因子：
64.5
作者：
FINLEY, D;OZKAYNAK, E;VARSHAVSKY, A
通讯作者：
VARSHAVSKY, A
影响因子：
3.3
作者：
Albig, AR;Decker, CJ
通讯作者：
Decker, CJ
影响因子：
3.2
作者：
FUGE, EK;BRAUN, EL;WERNERWASHBURNE, M
通讯作者：
WERNERWASHBURNE, M
影响因子：
56.9
作者：
GOEBL, MG;YOCHEM, J;BYERS, B
通讯作者：
BYERS, B