Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering.

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering.
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DOI:
10.1364/ol.33.002452
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发表时间:
2008-11-01
期刊:
影响因子:
3.6
通讯作者:
Wax A
Wax A
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Giacomelli MG;Chalut KJ;Ostrander JH;Wax A

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我们展示了一个逆光散射分析程序的基础上使用的T-矩阵方法作为光散射模型。我们使用角度分辨低相干干涉法(a/LCI)测量体外细胞单层散射的光,并将数据与T矩阵理论的预测进行比较。比较产生的测量等体积直径和纵横比的球状体细胞核的准确性相媲美的定量图像分析的固定和染色的样品。这些改进代表了a/LCI技术的重大升级,扩大了其适用的组织范围,并潜在地增加了其作为诊断工具的价值。
We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.
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