Lignocellulose degradation in Protaetia brevitarsis larvae digestive tract: refining on a tightly designed microbial fermentation production line.
Lignocellulose degradation in Protaetia brevitarsis larvae digestive tract: refining on a tightly designed microbial fermentation production line.
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DOI：
10.1186/s40168-022-01291-2
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发表时间：
2022-06-13
期刊：
影响因子：
15.5
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中科院分区：
文献类型：
--
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摘要金龟科短叶原蝇（Protaetia brevitarsis， PB）作为一种资源昆虫，其幼虫能有效地将腐烂的有机物转化为富含腐植酸的促进植物生长的杂草，并能产生健康、营养的昆虫蛋白质来源，近年来受到越来越多的研究关注。木质纤维素是PB幼虫饲料的主要成分，但PB基因组注释表明，PBL的糖活性酶不能完成木质纤维素的降解过程。因此，PBL高效降解木质纤维素的机理值得进一步研究。在此，我们使用宿主基因组和肠道宏基因组数据集来研究PBL的木质纤维素降解活性，并首次建立了肠道微生物基因和宿主肠道转录组基因的综合参考目录。我们描述了一个包含高度丰富和多样化的木质纤维素降解酶的基因库，并证明了PBL与其肠道细菌微生物群之间存在独特的团队合作，以实现有效的木质纤维素降解。PBL选择性地富集了主要来自厚壁菌门和拟杆菌门的木质纤维素降解微生物物种，这些微生物能够产生广泛的纤维素酶和半纤维素酶，因此在木质纤维素生物质降解中发挥了重要作用。此外，PBL微生物组中大多数与木质纤维素降解相关的模块序列都是新的。PBL通过其进化出的强口器、碱性中肠和温和稳定的后肠微环境为木质纤维素降解提供了有机功能互补，分别促进木质纤维素生物质的研磨、溶解和共生微生物发酵。该研究表明，PBL是研究木质纤维素降解的一个有前景的模型，可以为生物技术生物质转化行业提供丰富的新型酶和相关的木质纤维素降解菌株。PBL及其肠道共生菌群高效降解木质纤维素的独特团队合作将扩大对全息生物的认识，并开启全息生物理论的新开端。视频摘要在线版本包含补充材料，可在10.1186/s40168-022-01291-2获得。
The Scarabaeidae insect Protaetia brevitarsis (PB) has recently gained increasing research interest as a resource insect because its larvae can effectively convert decaying organic matter to plant growth-promoting frass with a high humic acid content and produce healthy, nutritional insect protein sources. Lignocellulose is the main component of PB larvae (PBL) feed, but PB genome annotation shows that PBL carbohydrate-active enzymes are not able to complete the lignocellulose degradation process. Thus, the mechanism by which PBL efficiently degrade lignocellulose is worthy of further study. Herein, we used combined host genomic and gut metagenomic datasets to investigate the lignocellulose degradation activity of PBL, and a comprehensive reference catalog of gut microbial genes and host gut transcriptomic genes was first established. We characterized a gene repertoire comprising highly abundant and diversified lignocellulose-degrading enzymes and demonstrated that there was unique teamwork between PBL and their gut bacterial microbiota for efficient lignocellulose degradation. PBL selectively enriched lignocellulose-degrading microbial species, mainly from Firmicutes and Bacteroidetes, which are capable of producing a broad array of cellulases and hemicellulases, thus playing a major role in lignocellulosic biomass degradation. In addition, most of the lignocellulose degradation-related module sequences in the PBL microbiome were novel. PBL provide organic functional complementarity for lignocellulose degradation via their evolved strong mouthparts, alkaline midgut, and mild stable hindgut microenvironment to facilitate lignocellulosic biomass grinding, dissolving, and symbiotic microbial fermentation, respectively. This work shows that PBL are a promising model to study lignocellulose degradation, which can provide highly abundant novel enzymes and relevant lignocellulose-degrading bacterial strains for biotechnological biomass conversion industries. The unique teamwork between PBL and their gut symbiotic bacterial microbiota for efficient lignocellulose degradation will expand the knowledge of holobionts and open a new beginning in the theory of holobionts. Video Abstract The online version contains supplementary material available at 10.1186/s40168-022-01291-2.
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影响因子：
3
作者：
Ham YK;Kim SW;Song DH;Kim HW;Kim IS
通讯作者：
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影响因子：
7.7
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影响因子：
6.3
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Bouchon, Didier
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4.3
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Huson DH;Beier S;Flade I;Górska A;El-Hadidi M;Mitra S;Ruscheweyh HJ;Tappu R
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Tappu R
影响因子：
6.3
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Gharechahi J;Salekdeh GH
通讯作者：
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