Increased vascular cell adhesion molecule-1 was associated with impaired endothelium-dependent relaxation of cerebral and carotid arteries in simulated microgravity rats.
Increased vascular cell adhesion molecule-1 was associated with impaired endothelium-dependent relaxation of cerebral and carotid arteries in simulated microgravity rats.
复制标题
在模拟微重力大鼠中，血管细胞粘附分子1的增加与脑动脉和颈动脉的内皮依赖性舒张受损有关。
DOI：
10.2170/physiolsci.rp010707
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发表时间：
2008-01
期刊：
影响因子：
--
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中科院分区：
文献类型：
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作者：
本研究的目的是探讨是否血管细胞粘附分子-1（VCAM-1）的表达上调，在3周模拟微重力大鼠脑动脉和颈动脉内皮依赖性舒张功能受损是否伴随着VCAM-1的表达。雄性SD大鼠随机分为对照组（CON）和后肢减重组（HU）。3周后，检测VCAM-1蛋白表达及基底动脉和颈总动脉血管舒张功能。免疫组化结果显示，VCAM-1阳性染色的血管内皮细胞在这些动脉从HU相比，CON大鼠。Western印迹分析证实了VCAM-1蛋白在HU大鼠的这些动脉中的表达上调。乙酰胆碱可引起所有动脉环的浓度依赖性舒张，但HU组基底动脉（P < 0.01）和颈总动脉（P < 0.05）的舒张幅度明显小于CON组。结果提示，模拟失重大鼠脑动脉和颈总动脉VCAM-1蛋白表达上调，VCAM-1蛋白表达上调可能与模拟失重大鼠血管内皮依赖性舒张功能受损有关。
The aim of the present study was to investigate whether an expression of vascular cell adhesion molecule-1 (VCAM-1) was upregulated in 3-week simulated microgravity rat cerebral and carotid arteries and whether impaired endothelium-dependent relaxation was concomitant with VCAM-1 expression. Male Sprague-Dawley rats were randomly divided into control (CON) and hindlimb unweighting (HU) groups. After 3 weeks, the expression of the VCAM-1 protein and the vasodilatation of the basilar artery and common carotid artery were determined. Immunohistochemical results revealed positive staining of VCAM-1 on endothelial cells in these arteries from HU compared with CON rats. Western blot analysis confirmed an upregulated expression of VCAM-1 protein in these arteries from HU rats. Acetylcholine induced concentration-dependent vasodilatation in all artery rings, but with significantly smaller amplitude in the basilar artery (P < 0.01) and the common carotid artery (P < 0.05) from HU than those from CON rats. The data suggested that the expression of VCAM-1 protein was upregulated in cerebral and common carotid arteries of simulated microgravity rats, and the upregulation of VCAM-1 may contribute to impaired endothelium-dependent relaxation in simulated microgravity rat vasculature.
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DOI：
--
发表时间：
2002-07
期刊：
Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology
影响因子：
--
作者：
M. Cirillo;N. D. De Santo;M. Heer;P. Norsk;B. Elmann-Larsen;L. Bellini;D. Stellato;C. Drummer
通讯作者：
M. Cirillo;N. D. De Santo;M. Heer;P. Norsk;B. Elmann-Larsen;L. Bellini;D. Stellato;C. Drummer
影响因子：
4.4
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H. Abdala-Valencia;J. Cook-Mills
DOI：
10.2170/jjphysiol.53.223
发表时间：
2003-06-01
期刊：
JAPANESE JOURNAL OF PHYSIOLOGY
影响因子：
--
作者：
Gotoh, TM;Fujiki, N;Morita, H
通讯作者：
Morita, H
影响因子：
3.3
作者：
Le-Ning Zhang;Li-fan Zhang;Jin Ma
通讯作者：
Le-Ning Zhang;Li-fan Zhang;Jin Ma
DOI：
--
发表时间：
2004-08
期刊：
Rinsho byori. The Japanese journal of clinical pathology
影响因子：
--
作者：
F. Ogiwara;Masafumi Takahashi;Uichi Ikeda
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F. Ogiwara;Masafumi Takahashi;Uichi Ikeda