Sequential changes in transforming growth factor (TGF)-β1 concentration in synovial fluid and mRNA expression of TGF-β1 receptors in chondrocytes after immobilization of rabbit knees
Sequential changes in transforming growth factor (TGF)-β1 concentration in synovial fluid and mRNA expression of TGF-β1 receptors in chondrocytes after immobilization of rabbit knees
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兔膝关节固定后滑液中转化生长因子（TGF）-β1浓度和软骨细胞中TGF-β1受体mRNA表达的连续变化
DOI：
10.1007/s007740170025
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发表时间：
2001
影响因子：
3.3
通讯作者：
T. Norimura
中科院分区：
文献类型：
--
作者：
R. Okazaki;A. Sakai;Y. Uezono;A. Ootsuyama;N. Kunugita;Toshitaka Nakamura;T. Norimura
我们以前曾报道过转化生长因子β 1（TGF-β 1）和碱性成纤维细胞生长因子（bFGF）联合应用可协同促进日本白色雄兔膝关节制动7 - 14天后软骨细胞的增殖。本研究以制动0-42 d的兔膝关节软骨细胞和关节液为实验材料，观察制动后关节液中TGF-β 1和bFGF浓度及软骨细胞中TGF-β 1受体I型（RI）和II型（RII）mRNA表达的变化。TGF-β 1和bFGF联合应用对制动7-14d的膝关节软骨细胞的增殖有协同作用。制动后7 - 28天滑液中TGF-β 1的浓度明显高于制动后2天的浓度（高达3.6倍）。RI和RII的mRNA在整个制动期间表达。bFGF在制动后2-7天维持在同一水平，此后逐渐下降。在软骨退变的早期，制动后14d，关节液中TGF-β 1和bFGF的浓度均较高，软骨细胞中TGF-β 1受体mRNA的表达仍保持不变。
Abstract We have previously reported that a combination of transforming growth factor (TGF)-β1 and basic fibroblast growth factor (bFGF) synergistically increases the proliferation of chondrocytes obtained from knee joint immobilized for 7–14 days in male Japanese white rabbits. In the present study, we performed experiments with chondrocytes and synovial fluid obtained from rabbit knees immobilized for 0–42 days, to clarify the sequential changes in TGF-β1 and bFGF concentrations in synovial fluid and the mRNA expression of TGF-β1 receptor type I (RI) and II (RII) in chondrocytes after immobilization. The combination of TGF-β1 and bFGF had a synergistic effect on the proliferation of chondrocytes obtained from knee joints immobilized for 7–14 days. The concentration of TGF-β1 in synovial fluid was significantly higher (up to 3.6-fold) at 7–28 days after immobilization compared with that at 2 days. The mRNA for RI and RII was expressed during the whole immobilization period. The concentration of bFGF was kept at the same level at 2–7 days after immobilization, and gradually decreased thereafter. In the early stages of degenerated cartilage, up to 14 days after immobilization, the concentrations of both TGF-β1 and bFGF were higher in the synovial fluid and mRNA expression of TGF-β1 receptors in chondrocytes was kept.
DOI：
10.1006/clin.1995.1114
发表时间：
1995-08
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Z. Szekanecz;G. Haines;L. Harlow;M. Shah;T. Fong;R. Fu;Samuel J.-W. Lin;Ghazi M. Rayan;Alisa E. Koch
通讯作者：
Z. Szekanecz;G. Haines;L. Harlow;M. Shah;T. Fong;R. Fu;Samuel J.-W. Lin;Ghazi M. Rayan;Alisa E. Koch
DOI：
10.1016/s0006-291x(88)80887-8
发表时间：
1988-10-31
影响因子：
3.1
作者：
CUEVAS, P;BURGOS, J;BAIRD, A
通讯作者：
BAIRD, A
转化生长因子-β1 对静息区软骨细胞的调节是由两条独立但相互作用的途径介导的。
DOI：
10.1016/s0167-4889(00)00030-6
发表时间：
2000
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Sylvia,VL;Schwartz,Z;Dean,DD;Boyan,BD
通讯作者：
Boyan,BD