Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.
Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.
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肺炎支原体感染过程中 A549 人肺泡上皮癌细胞的整体分泌组特征
DOI：
10.1186/1471-2180-14-27
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发表时间：
2014-02-07
期刊：
影响因子：
4.2
通讯作者：
Shan S
中科院分区：
文献类型：
--
作者：
Li S;Li X;Wang Y;Yang J;Chen Z;Shan S
背景：肺炎支原体（M.肺炎球菌（CAP）是所有年龄组的社区获得性肺炎（CAP）的主要病原体之一。早期寄主对M.肺炎杆菌感染依赖于具有各种生物活性的蛋白质的协同释放。然而，迄今为止还没有关于宿主对M的反应的分泌蛋白的全面分析。结果：采用液相色谱-串联质谱（LC-MS/MS）技术，对人肺泡上皮癌A549细胞分泌蛋白组进行了鉴定和分析。肺炎感染。共鉴定出256个蛋白质，其中113个差异表达（变化>1.5倍），其中9个仅在对照细胞中表达，10个仅在M. pneumoniae处理的细胞中有55个表达上调，39个表达下调。肺炎。通过RT-PCR和免疫印迹分析验证了一些鉴定的蛋白质的表达变化。分泌组数据的细胞定位分析显示，59.38%的蛋白被认为是“推定的分泌蛋白”。功能分析表明，这些蛋白质对M.肺炎链球菌感染主要与代谢过程、应激反应和免疫反应有关。我们进一步检测了临床样本中一种上调蛋白IL-33的水平。结果表明，肺炎支原体感染后血浆和支气管肺泡灌洗液（BALF）中IL-33水平显著升高;结论：本研究为肺炎支原体肺炎（MPP）患者分泌蛋白表达的变化提供了系统的信息。肺炎感染，这对于发现特异性生物标志物和药物干预的靶点是有用的。
Background:Mycoplasma pneumoniae (M. pneumoniae) is one of the major etiological agents for community-acquired pneumonia (CAP) in all age groups. The early host response to M. pneumoniae infection relies on the concerted release of proteins with various biological activities. However, no comprehensive analysis of the secretory proteins has been conducted to date regarding the host response upon M. pneumoniae infection.Results:We employed the liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based label-free quantitative proteomic technology to identify and characterize the members of the human alveolar epithelial carcinoma A549 cell secretome during M. pneumoniae infection. A total of 256 proteins were identified, with 113 being differentially expressed (>1.5-fold change), among which 9 were only expressed in control cells, 10 only in M. pneumoniae-treated cells, while 55 were up-regulated and 39 down-regulated by M. pneumoniae. The changed expression of some of the identified proteins was validated by RT-PCR and immunoblot analysis. Cellular localization analysis of the secretome data revealed 59.38% of the proteins were considered as "putative secretory proteins". Functional analysis revealed that the proteins affected upon M. pneumoniae infection were mainly related to metabolic process, stress response, and immune response. We further examined the level of one up-regulated protein, IL-33, in clinical samples. The result showed that IL-33 levels were significantly higher in the plasma and bronchoalveolar lavage fluid (BALF) of M. pneumoniae pneumonia (MPP) patients.Conclusions:The present study provided systematic information about the changes in the expression of secretory proteins during M. pneumoniae infection, which is useful for the discovery of specific biomarkers and targets for pharmacological intervention.
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影响因子：
3.4
作者：
Johansson, Carolina;Samskog, Jenny;Flensburg, John
通讯作者：
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影响因子：
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