Identification of Salivary Metabolic Signatures Associated with Primary Sjögren's Disease.
Identification of Salivary Metabolic Signatures Associated with Primary Sjögren's Disease.
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DOI：
10.3390/molecules28155891
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发表时间：
2023-08-05
期刊：
影响因子：
--
通讯作者：
Baleja JD
中科院分区：
文献类型：
--
作者：
Alt-Holland A;Huang X;Mendez T;Singh ML;Papas AS;Cimmino J;Bairos T;Tzavaras E;Foley E;Pagni SE;Baleja JD
干燥病（SjD）是第二大流行的自身免疫性疾病，涉及外分泌腺的慢性炎症。原发性SjD（pSjD）的正确诊断可以跨越许多年，因为疾病症状仅表现在唾液腺和泪腺破坏的晚期，并且共识诊断方法具有关键的灵敏度和选择性限制。使用核磁共振（NMR）光谱，我们确定了30名pSjD受试者和30名未患干燥病的参与者（非干燥对照组，NS-C）的未刺激唾液样本中代谢物的组成。在每个样本中定量34种代谢物，并对所有研究参与者（年龄23-78岁）的非标准化（浓度）和标准化代谢组学数据以及数据的年龄限制子集（年龄30-70岁）进行分析，同时在确定数据显著性时应用错误发现率校正。来自所有研究参与者和年龄限制子集的唾液样本的标准化数据表明，与NS-C组中的受试者相比，pSjD受试者中葡萄糖、甘油、牛磺酸和乳酸盐的水平显著增加，以及5-氨基戊酸盐、乙酸盐、丁酸盐和丙酸盐的水平显著降低。此外，与NS-C组相比，在来自pSjD组的唾液样品的标准化数据中，仅在年龄限制的亚组中发现胆碱的显著增加，并且仅在整个研究群体中发现岩藻糖的显著减少。来自所有研究参与者的唾液样本的代谢物浓度数据，但不是来自年龄限制的子集，表明与对照组相比，pSjD受试者的葡萄糖、甘油、牛磺酸和乳酸盐水平显著增加。该研究表明，NMR代谢组学可以用于定义与疾病状态相关的唾液代谢特征，并且可以有助于在临床上对pSjD受试者和未受这种疾病影响的受试者进行差异分析。
Sjögren’s disease (SjD) is the second most prevalent autoimmune disorder that involves chronic inflammation of exocrine glands. Correct diagnosis of primary SjD (pSjD) can span over many years since disease symptoms manifest only in advanced stages of salivary and lachrymal glandular destruction, and consensus diagnostic methods have critical sensitivity and selectivity limitations. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the composition of metabolites in unstimulated saliva samples from 30 pSjD subjects and 30 participants who do not have Sjögren’s disease (non-Sjögren’s control group, NS-C). Thirty-four metabolites were quantified in each sample, and analysis was conducted on both non-normalized (concentration) and normalized metabolomics data from all study participants (ages 23–78) and on an age-restricted subset of the data (ages 30–70) while applying false discovery rate correction in determining data significance. The normalized data of saliva samples from all study participants, and of the age-restricted subset, indicated significant increases in the levels of glucose, glycerol, taurine, and lactate, as well as significant decreases in the levels of 5-aminopentanoate, acetate, butyrate and propionate, in subjects with pSjD compared to subjects in the NS-C group. Additionally, a significant increase in choline was found only in the age-restricted subset, and a significant decrease in fucose was found only in the whole study population in normalized data of saliva samples from the pSjD group compared to the NS-C group. Metabolite concentration data of saliva samples from all study participants, but not from the age-restricted subset, indicated significant increases in the levels of glucose, glycerol, taurine, and lactate in subjects with pSjD compared to controls. The study showed that NMR metabolomics can be implemented in defining salivary metabolic signatures that are associated with disease status, and can contribute to differential analysis between subjects with pSjD and those who are not affected with this disease, in the clinic.
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DOI：
10.1016/j.jpba.2017.03.037
发表时间：
2017-06-05
影响因子：
3.4
作者：
Figueira, Joao;Gouveia-Figueira, Sandra;Ohman, Anders
通讯作者：
Ohman, Anders
影响因子：
81.5
作者：
Brito-Zeron, Pilar;Baldini, Chiara;Ramos-Casals, Manuel
通讯作者：
Ramos-Casals, Manuel
影响因子：
5.6
作者：
Hwang JS;Shin YJ
通讯作者：
Shin YJ
影响因子：
2.3
作者：
Giovelli RA;Santos MC;Serrano ÉV;Valim V
通讯作者：
Valim V
影响因子：
1.9
作者：
Herrala, Maria;Mikkonen, Jopi J. W.;Kullaa, Arja M.
通讯作者：
Kullaa, Arja M.