High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries.
High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries.
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DOI：
10.1038/s41598-017-14823-w
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发表时间：
2017-10-31
影响因子：
4.6
通讯作者：
Yang AS
中科院分区：
文献类型：
--
作者：
Chen IC;Chiu YK;Yu CM;Lee CC;Tung CP;Tsou YL;Huang YJ;Lin CL;Chen HS;Wang AH;Yang AS
甲型流感病毒(IAV)的大流行和暴发给人类社会带来了严峻的挑战。重组中和抗体的被动免疫治疗有可能减轻IAV感染的威胁。利用高通量中和抗体发现平台，我们从噬菌体展示的合成单链抗体文库中制备了人工抗血凝素(HA)IAV中和抗体，而不需要事先记忆抗体与抗原的相互作用，也不需要依赖于体内免疫系统产生高度特异性中和抗体所必需的亲和力成熟。人工抗HA抗体至少三分之二的表位群与天然保护性抗HA抗体相似，提供了天然抗体库中和抗体的替代品。随着设计和构建合成单链抗体文库的不断进步，这个技术平台不仅在缓解IAV大流行的威胁方面有用，而且在缓解其他新出现的病毒感染的威胁方面也是有用的。
Pandemic and epidemic outbreaks of influenza A virus (IAV) infection pose severe challenges to human society. Passive immunotherapy with recombinant neutralizing antibodies can potentially mitigate the threats of IAV infection. With a high throughput neutralizing antibody discovery platform, we produced artificial anti-hemagglutinin (HA) IAV-neutralizing IgGs from phage-displayed synthetic scFv libraries without necessitating prior memory of antibody-antigen interactions or relying on affinity maturation essential for in vivo immune systems to generate highly specific neutralizing antibodies. At least two thirds of the epitope groups of the artificial anti-HA antibodies resemble those of natural protective anti-HA antibodies, providing alternatives to neutralizing antibodies from natural antibody repertoires. With continuing advancement in designing and constructing synthetic scFv libraries, this technological platform is useful in mitigating not only the threats of IAV pandemics but also those from other newly emerging viral infections.
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DOI：
10.1155/2014/267594
发表时间：
2014
期刊：
Influenza research and treatment
影响因子：
--
作者：
Berry CM;Penhale WJ;Sangster MY
通讯作者：
Sangster MY
影响因子：
4.6
作者：
Chen HS;Hou SC;Jian JW;Goh KS;Shen ST;Lee YC;You JJ;Peng HP;Kuo WC;Chen ST;Peng MC;Wang AH;Yu CM;Chen IC;Tung CP;Chen TH;Ping Chiu K;Ma C;Yuan Wu C;Lin SW;Yang AS
通讯作者：
Yang AS
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
7.6
作者：
Laursen, Nick S.;Wilson, Ian A.
通讯作者：
Wilson, Ian A.
影响因子：
5.7
作者：
Chang, Hung-Ju;Jian, Jhih-Wei;Yang, An-Suei
通讯作者：
Yang, An-Suei