Human branch point consensus sequence is yUnAy.
Human branch point consensus sequence is yUnAy.
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DOI：
10.1093/nar/gkn073
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发表时间：
2008-04
影响因子：
14.9
通讯作者：
Ohno K
中科院分区：
文献类型：
--
作者：
Gao K;Masuda A;Matsuura T;Ohno K
酵母携带UACUAAC的严格保守的分支点序列（BPS），而人BPS是退化的并且不太好表征。迄今为止，人类共有BPS从未在体外进行过广泛探索。在这里，我们测序了367个克隆的LIPORT-PCR产物产生的52个内含子的20个人类管家基因。在367个克隆中，在181个克隆中观察到分支点处的错误掺入核苷酸，我们可以精确地定位分支点。分支点由92.3%的A、3.3%的C、1.7%的G和2.8%的U组成。我们的分析显示，人类共有BPS简单地是yUnAy，其中下划线是零位置的分支点，并且N-嘧啶（“y”）不如YunU和A那么保守。发现83%的克隆的分支点位于内含子3′端上游21-34个核苷酸处。我们还发现，多聚嘧啶道跨越下游的分支点的4-24个核苷酸。我们的分析表明，人类BPS的退行性比我们预期的更严重，并且人类BPS可能与多嘧啶段和/或其他剪接顺式元件结合被识别。
Yeast carries a strictly conserved branch point sequence (BPS) of UACUAAC, whereas the human BPS is degenerative and is less well characterized. The human consensus BPS has never been extensively explored in vitro to date. Here, we sequenced 367 clones of lariat RT-PCR products arising from 52 introns of 20 human housekeeping genes. Among the 367 clones, a misincorporated nucleotide at the branch point was observed in 181 clones, for which we can precisely pinpoint the branch point. The branch points were comprised of 92.3% A, 3.3% C, 1.7% G and 2.8% U. Our analysis revealed that the human consensus BPS is simply yUnAy, where the underlined is the branch point at position zero and the lowercase pyrimidines (‘y’) are not as well conserved as the uppercase U and A. We found that the branch points are located 21–34 nucleotides upstream of the 3′ end of an intron in 83% clones. We also found that the polypyrimidine tract spans 4–24 nucleotides downstream of the branch point. Our analysis demonstrates that the human BPSs are more degenerative than we have expected and that the human BPSs are likely to be recognized in combination with the polypyrimidine tract and/or the other splicing cis-elements.
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影响因子：
64.8
作者：
HORNIG, H;AEBI, M;WEISSMANN, C
通讯作者：
WEISSMANN, C
影响因子：
4.8
作者：
Garrey, Stephen M.;Voelker, Rodger;Berglund, J. Andrew
通讯作者：
Berglund, J. Andrew
影响因子：
3.5
作者：
Chavanas, S;Gache, Y;Meneguzzi, G
通讯作者：
Meneguzzi, G
影响因子：
5.3
作者：
HARTMUTH, K;BARTA, A
通讯作者：
BARTA, A
影响因子：
64.5
作者：
Berglund, JA;Chua, K;Rosbash, M
通讯作者：
Rosbash, M