Studies on the Energetics of Proaerolysin Secretion across the Outer Membrane of Aeromonas Species
Studies on the Energetics of Proaerolysin Secretion across the Outer Membrane of Aeromonas Species
复制标题
气单胞菌外膜分泌溶血素原的能量学研究
DOI：
--
复制
发表时间：
1997
影响因子：
4.8
通讯作者：
J. Buckley
中科院分区：
文献类型：
--
作者：
L. Letellier;S. P. Howard;J. Buckley
气单胞菌使用一般的分泌途径在分开的步骤中分泌通道形成蛋白气溶素原穿过它们的内膜和外膜。在这里，我们表明，治疗A。或A.在细胞中的ATP水平与对照分泌细胞中的水平没有不同的条件下，杀鲑素与质子载体羰基氰化物间氯苯腙阻断了运输的第二步，即从周质跨外膜分泌。在120 mV区域内观察到Δ Δ V的阈值，低于该阈值，两种物种的分泌均受到抑制。用砷酸盐处理细胞，降低ATP水平，但不影响Δ ATP，也减少了周质的分泌，这表明这一步骤需要ATP，而不需要Δ ATP。通过增加介质的渗透压也可以阻止跨外膜的分泌，即使细胞ATP水平和Δ ATP不受影响。这可能是由于内膜和外膜之间的某些必要联系被破坏。
Aeromonas spp. secrete the channel-forming protein proaerolysin across their inner and outer membranes in separate steps using the general secretion pathway. Here we show that treating A. hydrophila or A. salmonicida with the protonophore carbonyl cyanide m-chorophenyl hydrazone blocks the second step in transport, secretion across the outer membrane from the periplasm, under conditions where the ATP levels in the cell are no different than the levels in control, secreting cells. A threshold for ΔΨ was observed in the region of 120 mV, below which secretion by both species was inhibited. Treatment of cells with arsenate, which lowered ATP levels but did not affect ΔΨ, also reduced secretion from the periplasm, an indication that there is an ATP requirement for this step independent of the requirement for ΔΨ. Secretion across the outer membrane was also arrested by increasing the osmotic pressure of the medium, even though cellular ATP levels and ΔΨ were not affected. This may be due to disruption of some necessary association between the inner and outer membranes.
细胞质膜中的自激酶 EpsE 和 EpsL 之间的相互作用是霍乱弧菌胞外分泌所必需的。
DOI：
10.1002/j.1460-2075.1995.tb07155.x
发表时间：
1995
期刊：
The EMBO journal
影响因子：
--
作者：
Sandkvist,M;Bagdasarian,M;Howard,SP;DiRita,VJ
通讯作者：
DiRita,VJ
鼠伤寒沙门氏菌脂多糖易位的能量依赖性。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Marino,PA;Phan,KA;Osborn,MJ
通讯作者：
Osborn,MJ