Transgene Insertion Induced Dominant Male Sterility and Rescue of Male Fertility Using Round Spermatid Injection1
Transgene Insertion Induced Dominant Male Sterility and Rescue of Male Fertility Using Round Spermatid Injection1
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转基因插入诱导显性雄性不育并使用圆形精子细胞注射挽救雄性生育力1
DOI：
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发表时间：
2002
影响因子：
3.6
通讯作者：
M. Baetscher
中科院分区：
文献类型：
--
作者：
Xiaojuan Meng;H. Akutsu;K. Schoene;C. Reifsteck;E. P. Fox;S. Olson;H. Sariola;R. Yanagimachi;M. Baetscher
Abstract Transgene insertions in the mouse often cause mutations at chromosomal loci. Analysis of insertion mutations that cause male sterility may lead to the identification of novel molecular mechanisms implicated in male fertility. Here we show a line of transgenic mice with dominant inheritance of male sterility (DMS) that was found amid several lines that were normally fertile. Transgene-positive males from this line invariably were sterile, whereas transgenic females and transgene-negative male littermates were fertile. Histologic analysis and TUNEL staining for apoptotic cells in DMS testis showed spermatogenesis arrest at metaphase of meiosis I (M-I), accompanied by massive apoptosis of spermatocytes. Meiosis I arrest was incomplete, however, as small numbers of spermatids and spermatozoa were found. Both round spermatids and spermatozoa were evaluated for their permissiveness in the assisted reproductive technologies intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI). Surprisingly, ROSI but not ICSI gave live offspring, suggesting that mature sperm had deteriorated by the time of recovery from the epididymis. Mapping the transgene insertion by fluorescence in situ hybridization revealed a site on chromosome 14 D3-E1. Two candidate genes, GFRα2 and GnRH, that were previously mapped to that region and the functions of which in spermatogenesis are well established were not altered in DMS. As a consequence, positional cloning of the DMS locus will be essential to identify new molecules potentially involved in arrest at M-I. Furthermore, mice carrying this genetic trait might be useful for studies of assisted reproductive technologies and male contraceptives.
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DOI：
10.1073/pnas.95.24.14361
发表时间：
1998-11-24
影响因子：
11.1
作者：
Watson, ML;Zinn, AR;Moreadith, RW
通讯作者：
Moreadith, RW
影响因子：
4.6
作者：
Y. Kimura;Ryuzo Yanagimachi
通讯作者：
Y. Kimura;Ryuzo Yanagimachi
影响因子：
3.6
作者：
KIMURA, Y;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R
影响因子：
4.6
作者：
Warren G. Tourtellotte;Rakesh Nagarajan;Anthony Auyeung;Christina Mueller;Jeffrey Milbrandt
通讯作者：
Warren G. Tourtellotte;Rakesh Nagarajan;Anthony Auyeung;Christina Mueller;Jeffrey Milbrandt
由插入转基因引起的小鼠显性雄性不育。
DOI：
10.1073/pnas.88.22.10327
发表时间：
1991
影响因子：
11.1
作者：
Magram,J;Bishop,JM
通讯作者：
Bishop,JM