Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo.
Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo.
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DOI：
10.3389/fcell.2022.1015841
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发表时间：
2022
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞外囊泡（EV）在组织和细胞之间的通讯中发挥着重要作用。然而，体内特定组织分泌的EVs难以筛选和示踪，影响了病理生理条件下某些组织中EVs的功能研究。本研究构建了与mCherry蛋白系统共表达的Cre依赖性CD63flag-EGFP小鼠，可用于体内特定组织EV的分泌、运动和分选。该小鼠模型是研究不同病理生理条件下特定组织中EV的分泌量、靶组织和功能分子筛选的理想研究工具。此外，它为阐明分泌型EV在疾病发病机制中的作用机制提供了新的研究方法。
Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo. This mouse model is an ideal research tool for studying the secretion amount, target tissue, and functional molecule screening of EVs in specific tissues under different pathophysiological conditions. Moreover, it provides a new research method to clarify the mechanism of secreted EVs in the pathogenesis of the disease.
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影响因子：
3.7
作者：
Li J;Chen X;Yi J;Liu Y;Li D;Wang J;Hou D;Jiang X;Zhang J;Wang J;Zen K;Yang F;Zhang CY;Zhang Y
通讯作者：
Zhang Y
DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
影响因子：
11.8
作者：
Barman, Bahnisikha;Sung, Bong Hwan;Krystofiak, Evan;Ping, Jie;Ramirez, Marisol;Millis, Bryan;Allen, Ryan;Prasad, Nripesh;Chetyrkin, Sergei;Calcutt, M. Wade;Vickers, Kasey;Patton, James G.;Liu, Qi;Weaver, Alissa M.
通讯作者：
Weaver, Alissa M.
微泡介导的 microRNA-150 从单核细胞转移到内皮细胞可促进血管生成。
影响因子：
4.8
作者：
Li, Jing;Zhang, Yujing;Zhang, Chen-Yu
通讯作者：
Zhang, Chen-Yu
影响因子：
12.4
作者：
Maguire, Casey A.;Balaj, Leonora;Skog, Johan
通讯作者：
Skog, Johan