Cervical intraspinal microstimulation evokes robust forelimb movements before and after injury.
Cervical intraspinal microstimulation evokes robust forelimb movements before and after injury.
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DOI：
10.1088/1741-2560/10/3/036001
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发表时间：
2013-06
影响因子：
4
通讯作者：
Moritz CT
中科院分区：
文献类型：
--
作者：
Sunshine MD;Cho FS;Lockwood DR;Fechko AS;Kasten MR;Moritz CT
椎管内微刺激(ISMS)是一种很有前途的恢复神经损伤后瘫痪肢体的方法。颈椎和腰椎脊髓内的ISM能够在损伤前诱发各种高功能运动，但在颈髓损伤后诱发前肢运动的能力尚不清楚。在这里，我们检查了挫伤前和损伤后颈椎病激活的前肢运动和肌肉。我们记录了在损伤前以及中度C4/C5偏侧挫伤后3、6和9周，系统刺激啮齿动物颈脊髓所激活的前肢肌肉和诱发的运动。动物用异氟醚麻醉，以建立诱发运动的体位图，并量化颈椎节段C3和T1之间的诱发肌肉协同作用。当ISM被传递到颈髓时，在68%的测试位置观察到各种反应，其空间分布通常与运动神经元池的位置相对应。脊髓损伤对实现运动所需的刺激电流和可诱发运动的部位数量没有影响。分别在损伤后三周和六周观察到向伸展为主的运动和受限的肌肉协同作用的短暂转变。然而，在受伤后9周，ISMS诱发的模式与脊椎完整的动物相似。结果表明颈椎运动有可能在脊髓损伤后恢复手和手臂的功能。在与临床相关的持续性颈椎挫伤恢复的慢性阶段之前和期间，都可以诱发出强健的前肢运动。
Intraspinal microstimulation (ISMS) is a promising method for reanimating paralyzed limbs following neurological injury. ISMS within the cervical and lumbar spinal cord is capable of evoking a variety of highly-functional movements prior to injury, but the ability of ISMS to evoke forelimb movements after cervical spinal cord injury is unknown. Here we examine the forelimb movements and muscles activated by cervical ISMS both before and after contusion injury. We documented the forelimb muscles activated and movements evoked via systematic stimulation of the rodent cervical spinal cord both before injury and three, six and nine weeks following a moderate C4/C5 lateralized contusion injury. Animals were anesthetized with isoflurane to permit construction of somatotopic maps of evoked movements, and quantify evoked muscle synergies between cervical segments C3 and T1. When ISMS was delivered to the cervical spinal cord, a variety of responses were observed at 68% of locations tested, with a spatial distribution that generally corresponded to the location of motor neuron pools. Stimulus currents required to achieve movement and the number of sites where movements could be evoked were unchanged by spinal cord injury. A transient shift toward extension-dominated movements and restricted muscle synergies were observed at three and six weeks following injury, respectively. By nine weeks after injury, however, ISMS-evoked patterns were similar to spinally-intact animals. The results demonstrate the potential for cervical ISMS to reanimate hand and arm function following spinal cord injury. Robust forelimb movements can be evoked both before and during the chronic stages of recovery from a clinically-relevant and sustained cervical contusion injury.
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影响因子：
2.1
作者：
Giszter, SF;Loeb, E;Bizzi, E
通讯作者：
Bizzi, E
DOI：
10.1109/tnsre.2010.2052832
发表时间：
2011-02-01
影响因子：
4.9
作者：
Bamford, Jeremy A.;Putman, Charles T.;Mushahwar, Vivian K.
通讯作者：
Mushahwar, Vivian K.
DOI：
10.1109/86.830944
发表时间：
2000-03-01
期刊：
IEEE TRANSACTIONS ON REHABILITATION ENGINEERING
影响因子：
--
作者：
Mushahwar, VK;Horch, KW
通讯作者：
Horch, KW
影响因子：
2.5
作者：
Nakajima, K;Maier, MA;Lemon, RN
通讯作者：
Lemon, RN
影响因子：
5.3
作者：
McTigue, DM;Horner, PJ;Gage, FH
通讯作者：
Gage, FH