Cerebral angiogenesis induced by growth factors: intravital microscopic studies using models.
Cerebral angiogenesis induced by growth factors: intravital microscopic studies using models.
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生长因子诱导的脑血管生成：使用模型的活体显微镜研究。
DOI：
--
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发表时间：
2003
影响因子：
2.1
通讯作者：
H. Niimi
中科院分区：
文献类型：
--
作者：
H. Niimi
药物诱导血管生成是一种非手术治疗脑血管功能不全的新方法。研究在体脑肿瘤组织血管生成和微血管灌流的动态过程是一项紧迫的任务。本研究涉及活体脑内新生毛细血管的微循环方面。利用碱性成纤维细胞生长因子(BFGF)和血小板衍生生长因子(PDGF)等生长因子诱导小鼠脑内新生血管，建立了一种新的脑血管生成模型。通过在荧光显微镜下直接观察新生毛细血管微循环，测量新生毛细血管密度、直径和红细胞速度，以评价孵育后天数对新生毛细血管的发育和重建的影响。通过测量直径和红细胞速度的变化来检测局部应用乙酰胆碱(ACh)对新毛细血管的反应。结果表明，在培养第28天，PDGF诱导的新生毛细血管对ACh的反应是扩张的，而bFGF诱导的新生毛细血管不是扩张的。此外，根据肿瘤组织的荧光免疫组织化学图像，用激光共聚焦显微镜观察新的毛细血管周细胞。新生毛细血管内可见数个抗NG_2抗体染色的周细胞。提示这些周细胞可能在新生毛细血管中募集，以调节血流，而不需要血管平滑肌。
Pharmacological induction of angiogenesis is a new treatment of cerebrovascular insufficiency without surgical treatment. It is an urgent task to investigate the dynamic process of angiogenesis and of the microvascular perfusion of the cerebral neoplastic tissue in vivo. The present study is concerned with microcirculatory aspects of cerebral neocapillaries in vivo. A novel model of cerebral angiogenesis was developed by inducing cerebral neocapillaries in mice using growth factors such as basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF). By a direct observation of the neocapillary microcirculation under a fluorescence videomicroscope, the neocapillary density, diameter and red cell velocity were measured to evaluate the development and remodeling of the neocapillaries with the number of days after incubation. The neocapillary response to topically applied acetylcholine (ACh) was examined by measuring changes in the diameter and red cell velocity. It was shown that PDGF-induced neocapillaries was dilated in response to ACh on day 28 after incubation, while bFGF-induced neocapillaries was not. Furthermore, the neocapillary pericytes were observed using confocal laser microscopy, based on the fluorescence immunohistochemical images of the neoplastic tissue. Several pericytes, stained with anti-NG_2, appeared in the neocapillaries. It was suggested that these pericytes might be recruited in the neocapillaries to regulate blood flow without vascular smooth muscle.
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影响因子：
11.2
作者：
M. Leunig;F. Yuan;M. Menger;Y. Boucher;A. Goetz;K. Messmer;R. Jain
通讯作者：
M. Leunig;F. Yuan;M. Menger;Y. Boucher;A. Goetz;K. Messmer;R. Jain
发育良好的犬冠状侧支血管的内皮功能。
DOI：
10.1152/ajpheart.1993.264.2.h567
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Altman,J;Dulas,D;Pavek,T;Laxson,DD;Homans,DC;Bache,RJ
通讯作者：
Bache,RJ
新发育的冠状侧支血管的血管舒缩反应。
DOI：
10.1152/ajpheart.1996.271.2.h490
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
Kinn,JW;Altman,JD;Chang,MW;Bache,RJ
通讯作者：
Bache,RJ
小鼠血管生成血管的定量和生理特征：碱性成纤维细胞生长因子、血管内皮生长因子/血管通透性因子和宿主微环境的影响。
DOI：
--
发表时间：
1996
期刊：
The American journal of pathology.
影响因子：
--
作者：
Dellian,M;Witwer,BP;Salehi,HA;Yuan,F;Jain,RK
通讯作者：
Jain,RK
胰岛移植微血管的组织发生和超微结构。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Vajkoczy,P;Olofsson,AM;Lehr,HA;Leiderer,R;Hammersen,F;Arfors,KE;Menger,MD
通讯作者：
Menger,MD