Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals.
Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals.
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DOI：
10.1083/jcb.130.1.169
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发表时间：
1995-07
影响因子：
7.8
通讯作者：
SMITH, TR
中科院分区：
文献类型：
--
作者：
LI, WC;KUSZAK, JR;DUNN, K;WANG, RR;MA, WC;WANG, GM;SPECTOR, A;LEIB, M;COTLIAR, AM;WEISS, M;ESPY, J;HOWARD, G;FARRIS, RL;AURAN, J;DONN, A;HOFELDT, A;MACKAY, C;MERRIAM, J;MITTL, R;SMITH, TR
白内障是世界上许多发展中国家导致失明的一种主要眼病。众所周知，各种因素，如氧化应激、紫外线和其他有毒物质，都可以在体内和体外诱导白内障的形成。然而，这种诱导的共同细胞基础以前还没有被认识到。目前对晶状体上皮细胞活性的研究表明，这是一个普遍的机制。对20例年龄在12~94岁的白内障患者的晶状体上皮细胞进行末端脱氧核苷酸转移酶(TDT)标记和DNA片段化分析，发现所有患者的晶状体上皮细胞凋亡率在4.4%~41.8%之间。相比之下，在8个年龄相当的正常人晶状体中，几乎没有观察到凋亡的上皮细胞。我们认为，白内障患者在发病时可能存在对氧化应激和紫外线等因素的防御系统不足。这种应激可以触发晶状体上皮细胞的凋亡，进而可能引发白内障的发生。为了验证这一假设，这里还证明了以前在一些白内障患者中发现的浓度的过氧化氢同时诱导晶状体上皮细胞凋亡和皮质混浊。此外，诱导凋亡的晶状体上皮细胞的时间和空间分布先于晶状体混浊的发生。这些结果提示晶状体上皮细胞的凋亡可能是非先天性白内障形成的共同细胞学基础。
Cataract is a major ocular disease that causes blindness in many developing countries of the world. It is well established that various factors such as oxidative stress, UV, and other toxic agents can induce both in vivo and in vitro cataract formation. However, a common cellular basis for this induction has not been previously recognized. The present study of lens epithelial cell viability suggests such a general mechanism. When lens epithelial cells from a group of 20 cataract patients 12 to 94 years old were analyzed by terminal deoxynucleotidyl transferase (TdT) labeling and DNA fragmentation assays, it was found that all of these patients had apoptotic epithelial cells ranging from 4.4 to 41.8%. By contrast, in eight normal human lenses of comparable age, very few apoptotic epithelial cells were observed. We suggest that cataract patients may have deficient defense systems against factors such as oxidative stress and UV at the onset of the disease. Such stress can trigger lens epithelial cell apoptosis that then may initiate cataract development. To test this hypothesis, it is also demonstrated here that hydrogen peroxide at concentrations previously found in some cataract patients induces both lens epithelial cell apoptosis and cortical opacity. Moreover, the temporal and spatial distribution of induced apoptotic lens epithelial cells precedes development of lens opacification. These results suggest that lens epithelial cell apoptosis may be a common cellular basis for initiation of noncongenital cataract formation.
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影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
DOI：
10.1016/0006-291x(66)90447-5
发表时间：
1966-01-01
影响因子：
3.1
作者：
DENHARDT, DT
通讯作者：
DENHARDT, DT
影响因子：
3.4
作者：
FUKUI, HN
通讯作者：
FUKUI, HN
DOI：
10.1073/pnas.80.7.2044
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GARNER, WH;GARNER, MH;SPECTOR, A
通讯作者：
SPECTOR, A