Homologs of the small RNA SgrS are broadly distributed in enteric bacteria but have diverged in size and sequence.
Homologs of the small RNA SgrS are broadly distributed in enteric bacteria but have diverged in size and sequence.
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DOI：
10.1093/nar/gkp501
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发表时间：
2009-09
影响因子：
14.9
通讯作者：
Vanderpool CK
中科院分区：
文献类型：
--
作者：
Horler RS;Vanderpool CK
大肠杆菌中的糖磷酸盐胁迫是由转录调控因子SgrR和小RNA SgrS感知和管理的。SgrS是一种双重功能RNA，它对mRNA靶点进行碱基配对依赖性调控，并编码一种小蛋白SgrT。分析了SgrR的同源物的基因同一性和同源性，以确定那些可能在功能上相似的同源物。这22个SgrR同源物被用来手动定位功能类似SgrS的邻近srna。SgrS同源物与大肠杆菌SgrS同源物在序列上几乎没有相同之处，但大多数同源物具有相同的结构特征。SgrS同源物最保守的特征是碱基配对区，而变化最大的特征是sgrt编码序列。对不同生物中SgrS:ptsG mRNA对相互作用的预测分析揭示了碱基配对相互作用模式的有趣差异。互补中断区域较多的RNA对的G:C碱基对比例高于互补连续区域较长的RNA对。这组同源sRNAs及其靶点的鉴定为进一步阐明SgrS调控的分子要求奠定了基础。
Sugar phosphate stress in Escherichia coli is sensed and managed by the transcriptional regulator SgrR and the small RNA (sRNA) SgrS. SgrS is a dual function RNA that performs base pairing-dependent regulation of mRNA targets and encodes a small protein, SgrT. Homologs of SgrR were analyzed for gene synteny and inter-homolog identity to identify those that are likely to be functionally analogous. These 22 SgrR homologs were used to manually locate adjacent sRNAs functionally analogous to SgrS. SgrS homologs shared little sequence identity with E. coli SgrS, but most shared several structural features. The most conserved feature of SgrS homologs was the base pairing region while the most variable feature was the sgrT-coding sequence. Analyses of predicted interactions between SgrS:ptsG mRNA pairs in different organisms revealed interesting differences in the patterns of base pairing interactions. RNA pairs with more interrupted regions of complementarity had a higher proportion of G:C base pairs than those with longer contiguous stretches of complementarity. The identification of this set of homologous sRNAs and their targets sets the stage for future studies to further elucidate the molecular requirements for regulation by SgrS.
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影响因子：
4.8
作者：
Liu, JY;Miller, PF;Olson, ER
通讯作者：
Olson, ER
影响因子：
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作者：
Argaman, L;Altuvia, S
通讯作者：
Altuvia, S
DOI：
10.1073/pnas.032066599
发表时间：
2002-04-02
影响因子：
11.1
作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
14.9
作者：
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