Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins.
Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins.
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DOI：
10.1083/jcb.96.1.230
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发表时间：
1983-01
期刊：
影响因子：
--
通讯作者：
Ma A
中科院分区：
文献类型：
--
作者：
Hubbard AL;Ma A
分离大鼠肝细胞质膜(Hubbard，A.L.，D.A.Wall，and A.Ma，1983，J.Cell Biol.，96：217-229)，发现大鼠肝细胞质膜中含有许多细丝，这些细丝既与横向表面的桥粒有关，也与构成三个结构域(横向[LS]、胆小管[BC]和肝窦[SF])的细胞质有关。将质膜暴露在碱性介质(最高pH为11)中，可导致可识别的细丝丢失，而不会损失结构域形态或膜酶活性。对对照质膜和碱抽提质膜中可溶组分的电泳分析表明，对照质膜中43、52和56kdalton处的三种主要多肽已被碱处理(PH 11)释放，并可在上清液中定量回收。将43 kdalton组分与肌动蛋白(α)和刷状缘肌动蛋白(β，γ)进行比较，确定其为胞质肌动蛋白。52kdalton和56kdalton多肽被鉴定为张力细丝成分，因为它们的溶解性和它们从存在于碱性提取物中的单体重新组装成9.5 nm细丝的能力。
Rat liver plasma membranes were isolated as presented in the preceding paper (Hubbard, A. L., D. A. Wall, and A. Ma., 1983, J. Cell Biol., 96: 217-229) and found to contain many filaments associated both with desmosomes along the lateral surface and with the cytoplasmic aspects of membranes comprising each of the three domains (lateral [LS], bile canalicular [BC] and sinusoidal [SF] ). Exposure of the plasma membranes to alkaline media (up to pH 11) resulted in loss of recognizable filaments without loss of domain morphology or membrane enzyme activities. Electrophoretic analysis of solubilized components from control and alkaline-extracted plasma membranes revealed that three major polypeptides present at 43, 52, and 56 kdaltons in the control had been released by alkaline treatment (pH 11) and could be quantitatively recovered in the supernate. The 43-kdalton component was identified as cytoplasmic actin by comparison of its tryptic 125I- peptide map to those of muscle (alpha) and brush border (beta, gamma) actins. The 52- and 56-kdalton polypeptides were identified as tonofilament components by their solubility properties and their ability to reassemble into 9.5-nm filaments from monomers present in an alkaline extract.
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DOI：
10.1083/jcb.93.1.97
发表时间：
1982-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fujiki Y;Hubbard AL;Fowler S;Lazarow PB
通讯作者：
Lazarow PB
影响因子：
64.5
作者：
CRAIG, SW;POWELL, LD
通讯作者：
POWELL, LD
影响因子：
7.8
作者：
FRANKE, WW;SCHMID, E;KEENAN, TW
通讯作者：
KEENAN, TW
DOI：
10.1139/o76-068
发表时间：
1976-01-01
期刊：
CANADIAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GURD, JW;EVANS, WH
通讯作者：
EVANS, WH
DOI：
10.1016/0006-291x(75)90782-2
发表时间：
1975-01-01
影响因子：
3.1
作者：
SCHMIDTULLRICH, R;VERMA, SP;WALLACH, DFH
通讯作者：
WALLACH, DFH