Baicalin increases developmental competence of mouse embryos in vitro by inhibiting cellular apoptosis and modulating HSP70 and DNMT expression.
Baicalin increases developmental competence of mouse embryos in vitro by inhibiting cellular apoptosis and modulating HSP70 and DNMT expression.
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黄芩苷通过抑制细胞凋亡和调节 HSP70 和 DNMT 表达来提高小鼠胚胎的体外发育能力
DOI：
10.1262/jrd.2016-047
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发表时间：
2016-12-20
期刊：
影响因子：
--
通讯作者：
Tian W
中科院分区：
文献类型：
--
作者：
Qi X;Li H;Cong X;Wang X;Jiang Z;Cao R;Tian W
黄芩在中药中被有效地用于预防流产。然而，关于其作用机制的资料很少。本研究以小鼠为模型，揭示黄芩主要有效成分黄芩苷对体外胚胎发育能力的促进作用。小鼠原核胚胎在添加（0、2、4和8 μg/ml）黄芩苷的KSOM培养基中培养。结果表明，体外培养条件显著降低囊胚发育率和囊胚质量，可能与细胞应激和凋亡增加有关。黄芩苷（4µg/ml）显著提高体外培养小鼠胚胎2细胞和4细胞的分裂率、桑葚胚发育率、囊胚发育率和细胞数量。黄芩苷通过降低体外培养囊胚中HSP70、CASP3、BAX的表达和Bcl-2的表达，提高Gja1、Cdh1、Bcl-2和Dnmt3a基因的表达，降低Dnmt1基因的表达，降低细胞应激和细胞凋亡。综上所述，黄芩苷通过抑制细胞凋亡和HSP70的表达，以及改善DNA甲基化来提高体外培养小鼠胚胎的发育能力。
Scutellaria baicalensis has been effectively used in Chinese traditional medicine to prevent miscarriages. However, little information is available on its mechanism of action. This study is designed specifically to reveal how baicalin, the main effective ingredient of S. baicalensis, improves developmental competence of embryos in vitro, using the mouse as a model. Mouse pronuclear embryos were cultured in KSOM medium supplemented with (0, 2, 4 and 8 μg/ml) baicalin. The results demonstrated that in vitro culture conditions significantly decreased the blastocyst developmental rate and blastocyst quality, possibly due to increased cellular stress and apoptosis. Baicalin (4 µg/ml) significantly increased 2- and 4-cell cleavage rates, morula developmental rate, and blastocyst developmental rate and cell number of in vitro-cultured mouse embryos. Moreover, baicalin increased the expression of Gja1, Cdh1, Bcl-2, and Dnmt3a genes, decreased the expression of Dnmt1 gene, and decreased cellular stress and apoptosis as it decreased the expression of HSP70, CASP3, and BAX and increased BCL-2 expression in blastocysts cultured in vitro. In conclusion, baicalin improves developmental competence of in vitro-cultured mouse embryos through inhibition of cellular apoptosis and HSP70 expression, and improvement of DNA methylation.
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影响因子：
3.7
作者：
Cánepa MJ;Ortega NM;Monteleone MC;Mucci N;Kaiser GG;Brocco M;Mutto A
通讯作者：
Mutto A
影响因子：
3.6
作者：
Chung, YG;Ratnam, S;Latham, KE
通讯作者：
Latham, KE
影响因子：
3.6
作者：
Rizos, D;Gutiérrez-Adán, A;Lonergan, P
通讯作者：
Lonergan, P
DNA甲基化重编程相关基因的表达模式与猪合子基因组激活后克隆胚胎的发育能力相关
影响因子：
1.2
作者：
Huan, Yanjun;Wang, Hongmei;He, Hongbin
通讯作者：
He, Hongbin
影响因子：
2.8
作者：
Lonergan, P;Fair, T;Evans, ACO
通讯作者：
Evans, ACO