Clinical application of plasma thermograms. Utility, practical approaches and considerations.

Clinical application of plasma thermograms. Utility, practical approaches and considerations.
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DOI:
10.1016/j.ymeth.2014.10.030
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发表时间:
2015-04
期刊:
影响因子:
4.8
通讯作者:
Chaires, Jonathan B.
Chaires, Jonathan B.
中科院分区:
生物学3区
文献类型:
--
作者:
Garbett, Nichola C.;Mekmaysy, Chongkham S.;DeLeeuw, Lynn;Chaires, Jonathan B.

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血浆差示扫描量热法 (DSC) 研究是 DSC 生物流体分析临床应用新兴领域的一部分。对健康个体和患有各种疾病的患者血浆的 DSC 分析揭示了与患者临床状态相关的主要血浆蛋白的热分布变化。 DSC 对蛋白质浓度、蛋白质与其他蛋白质或配体的相互作用或其共价修饰的敏感性是 DSC 分析潜在用途的基础。越来越多的文献证明了临床 DSC 分析在一系列生物流体和多种疾病环境中的多功能性和性能。本文介绍了等离子体 DSC 分析的原理、实践和挑战。
Differential scanning calorimetry (DSC) studies of blood plasma are part of an emerging area of the clinical application of DSC to biofluid analysis. DSC analysis of plasma from healthy individuals and patients with various diseases has revealed changes in the thermal profiles of the major plasma proteins associated with the clinical status of the patient. The sensitivity of DSC to the concentration of proteins, their interactions with other proteins or ligands, or their covalent modifications underlies the potential utility of DSC analysis. A growing body of literature has demonstrated the versatility and performance of clinical DSC analysis across a range of biofluids and in a number of disease settings. The principles, practice and challenges of DSC analysis of plasma are described in this article.
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