Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells.
Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells.
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DOI：
10.1016/j.exer.2010.04.004
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发表时间：
2010-07
影响因子：
3.4
通讯作者：
Shestopalov, Valery I.
中科院分区：
文献类型：
--
作者：
Crabb, John W.;Yuan, Xianglin;Dvoriantchikova, Galina;Ivanov, Dmitry;Crabb, John S.;Shestopalov, Valery I.
关键词：
对单侧实验性青光眼大鼠视网膜神经节细胞（RGCs）进行定量蛋白质组学分析。在持续升高的眼内压8周后，通过免疫淘洗从22只动物中分离RGCs。通过LC MS/MS iTRAQ技术定量蛋白质。在定量的268种蛋白质中，约8%在青光眼RGC中出现升高，约13%降低。电压依赖性阴离子通道蛋白2、醛糖还原酶和泛素在显著升高的蛋白质中，而胸腺素原在显著降低的蛋白质中。结果证明了鉴定体内纯化的胶质瘤和对照RGCs之间蛋白质表达的全局蛋白质组差异的可行性。
Quantitative proteomic analysis was pursued of retinal ganglion cells (RGCs) from rats with unilateral experimental glaucoma. RGCs were isolated from 22 animals by immunopanning after 8 weeks of sustained elevated intraocular pressure. Proteins were quantified by LC MS/MS iTRAQ technology. Of the 268 proteins quantified, ~8% appeared elevated and ~13% decreased in glaucomatous RGCs. Voltage-dependent anion channel protein 2, aldose reductase, and ubiquitin were among the significantly elevated proteins while prothymosin was among the significantly decreased. The results demonstrate the feasibility of identifying global proteomic differences in protein expression between in vivo purified glaucomatous and control RGCs.
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影响因子：
7
作者：
Zhan, Xianquan;Du, Yunpeng;Crabb, John W.
通讯作者：
Crabb, John W.
DOI：
10.1073/pnas.222551899
发表时间：
2002-11-12
影响因子：
11.1
作者：
Crabb, JW;Miyagi, M;Hollyfield, JG
通讯作者：
Hollyfield, JG
影响因子：
4.8
作者：
Bhattacharya, SK;Rockwood, EJ;Crabb, JW
通讯作者：
Crabb, JW
影响因子：
4.1
作者：
Quigley, HA;Broman, AT
通讯作者：
Broman, AT
影响因子：
7
作者：
Ng, Kwok-Peng;Gugiu, Bogdan;Crabb, John W.
通讯作者：
Crabb, John W.