Redundant mechanisms for regulation of midline crossing in Drosophila.
Redundant mechanisms for regulation of midline crossing in Drosophila.
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DOI：
10.1371/journal.pone.0003798
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Gilestro GF
中科院分区：
文献类型：
--
作者：
Gilestro GF
在发育过程中，所有神经元都必须决定是否穿过纵向中线投射到身体的对侧。在脊椎动物和无脊椎动物中，通过干扰Robo信号传导来实现对交叉的调节，在果蝇中通过受体的分选和降解，或者在脊椎动物中通过抑制其排斥活性。在这里，我表明，在果蝇的第二种机制的监管存在，是独立于排序。使用体外和体内测定，我映射的区域的机器人是足够的，需要它的相互作用与通讯，其分选受体。通过修饰该区域，我产生了新形式的Robo，它们在体外和体内对Comm分选不敏感，但仍然能够在体内正常翻译排斥活性。使用同源重组的基因靶向，我创建了分选缺陷的robo的新条件等位基因（roboSD）。令人惊讶的是，这些修饰的蛋白质的表达结果在表型正常的苍蝇，揭示了一个排序独立的调节机制。
During development, all neurons have to decide on whether to cross the longitudinal midline to project on the contralateral side of the body. In vertebrates and invertebrates regulation of crossing is achieved by interfering with Robo signalling either through sorting and degradation of the receptor, in flies, or through silencing of its repulsive activity, in vertebrates. Here I show that in Drosophila a second mechanism of regulation exists that is independent from sorting. Using in vitro and in vivo assays I mapped the region of Robo that is sufficient and required for its interaction with Comm, its sorting receptor. By modifying that region, I generated new forms of Robo that are insensitive to Comm sorting in vitro and in vivo, yet still able to normally translate repulsive activity in vivo. Using gene targeting by homologous recombination I created new conditional alleles of robo that are sorting defective (roboSD). Surprisingly, expression of these modified proteins results in phenotypically normal flies, unveiling a sorting independent mechanism of regulation.
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