Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn's disease and healthy individuals.
Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn's disease and healthy individuals.
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DOI：
10.1007/s11306-014-0677-3
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发表时间：
2015
期刊：
影响因子：
3.6
通讯作者：
Nielsen, Ole Haagen
中科院分区：
文献类型：
--
作者：
Bjerrum, Jacob Tveiten;Wang, Yulan;Hao, Fuhua;Coskun, Mehmet;Ludwig, Christian;Guenther, Ulrich;Nielsen, Ole Haagen
本研究通过对健康受试者、活动期或静止期溃疡性结肠炎(UC)和克罗恩病(CD)患者粪便提取液的光谱学代谢谱分析，证实光谱学作为一种非侵入性诊断工具的潜在用途，并描述炎症性肠病(IBD)的粪便代谢组。用1H核磁共振波谱(Bruker 600 MHz，Bruker BioSpin，Rheinstetten，Rheinstetten)对113例个体(UC 48例，CD 44例，对照组21例)的大便标本进行分析。数据处理采用主成分分析和正交投影潜在结构判别分析，使用Simca-P和MatLab软件。在代谢谱上发现了显着的差异，这使得区分活动期IBD和对照组以及UC和CD成为可能。具有不同能力的代谢物主要属于一系列氨基酸、微生物区系相关的短链脂肪酸和乳酸，表明正常细菌生态的炎症驱动的吸收不良和生态失调。然而，切除肠道手术和抗肿瘤坏死因子-α抗体治疗的患者消除了UC和CD的区别能力。这项研究结果表明，粪便提取物的1H核磁共振波谱是一种潜在的非侵入性诊断工具，并能够表征与吸收不良和生物失调相关的炎症驱动的代谢变化。肠道手术和药物治疗将在未来的研究中考虑在内，因为这似乎是歧视过程中的重要因素。本文的在线版本(doi：10.1007/s11306-0140677-3)包含补充材料，授权用户可以使用。
This study employs spectroscopy-based metabolic profiling of fecal extracts from healthy subjects and patients with active or inactive ulcerative colitis (UC) and Crohn’s disease (CD) to substantiate the potential use of spectroscopy as a non-invasive diagnostic tool and to characterize the fecal metabolome in inflammatory bowel disease (IBD). Stool samples from 113 individuals (UC 48, CD 44, controls 21) were analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy (Bruker 600 MHz, Bruker BioSpin, Rheinstetten, Germany). Data were analyzed with principal component analysis and orthogonal-projection to latent structure-discriminant analysis using SIMCA-P + 12 and MATLAB. Significant differences were found in the metabolic profiles making it possible to differentiate between active IBD and controls and between UC and CD. The metabolites holding differential power primarily belonged to a range of amino acids, microbiota-related short chain fatty acids, and lactate suggestive of an inflammation-driven malabsorption and dysbiosis of the normal bacterial ecology. However, removal of patients with intestinal surgery and anti-TNF-α antibody treatment eliminated the discriminative power regarding UC versus CD. This study consequently demonstrates that 1H NMR spectroscopy of fecal extracts is a potential non-invasive diagnostic tool and able to characterize the inflammation-driven changes in the metabolic profiles related to malabsorption and dysbiosis. Intestinal surgery and medication are to be accounted for in future studies, as it seems to be factors of importance in the discriminative process. The online version of this article (doi:10.1007/s11306-014-0677-3) contains supplementary material, which is available to authorized users.
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影响因子：
3.6
作者：
Bjerrum, Jacob Tveiten;Rantalainen, Mattias;Wang, Yulan;Olsen, Jorgen;Nielsen, Ole Haagen
通讯作者：
Nielsen, Ole Haagen
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.4
作者：
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