The unusual cell wall of the Lyme disease spirochaete Borrelia burgdorferi is shaped by a tick sugar.
The unusual cell wall of the Lyme disease spirochaete Borrelia burgdorferi is shaped by a tick sugar.
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DOI：
10.1038/s41564-021-01003-w
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发表时间：
2021-12
影响因子：
28.3
通讯作者：
Jutras BL
中科院分区：
文献类型：
--
作者：
DeHart TG;Kushelman MR;Hildreth SB;Helm RF;Jutras BL
肽聚糖--由肽连接的聚糖的网状囊--是细菌细胞壁的主要成分。肽聚糖提供结构强度，保护细胞免受渗透压影响并有助于形状。所有细菌聚糖都是N-乙酰葡糖胺（GlcNAc）β-（1-4）-连接N-乙酰胞壁酸（MurNAc）的重复二糖。伯氏疏螺旋体，蜱传莱姆病病原体，产生聚糖链，其中MurNAc偶尔被未知的糖取代。核磁共振、液相色谱-质谱和遗传分析表明，B。burgdorferi产生含有GlcNAc-GlcNAc的聚糖。这种不寻常的二糖是壳二糖，是其几丁质蜱载体的一种成分。壳二糖营养缺陷型的突变细菌具有改变的形态、降低的运动性和细胞包膜缺陷，这可能是由于产生比野生型细菌更硬的肽聚糖。我们认为B的肽聚糖。burgdorferi可能通过适应蜱虫载体的专性寄生而进化，导致生物物理细胞壁改变以承受与扭转运动相关的非典型扭矩。蜱虫壳二糖被增选用于构建莱姆病病原体伯氏疏螺旋体的细胞壁。
Peptidoglycan—a mesh sac of glycans that are linked by peptides—is the main component of bacterial cell walls. Peptidoglycan provides structural strength, protects cells from osmotic pressure and contributes to shape. All bacterial glycans are repeating disaccharides of N-acetylglucosamine (GlcNAc) β-(1–4)-linked to N-acetylmuramic acid (MurNAc). Borrelia burgdorferi, the tick-borne Lyme disease pathogen, produces glycan chains in which MurNAc is occasionally replaced with an unknown sugar. Nuclear magnetic resonance, liquid chromatography–mass spectroscopy and genetic analyses show that B. burgdorferi produces glycans that contain GlcNAc–GlcNAc. This unusual disaccharide is chitobiose, a component of its chitinous tick vector. Mutant bacteria that are auxotrophic for chitobiose have altered morphology, reduced motility and cell envelope defects that probably result from producing peptidoglycan that is stiffer than that in wild-type bacteria. We propose that the peptidoglycan of B. burgdorferi probably evolved by adaptation to obligate parasitization of a tick vector, resulting in a biophysical cell-wall alteration to withstand the atypical torque associated with twisting motility. Tick chitobiose is co-opted to build the cell wall of Lyme disease pathogen Borrelia burgdorferi.
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影响因子：
6.7
作者：
Gupta A;Arora G;Rosen CE;Kloos Z;Cao Y;Cerny J;Sajid A;Hoornstra D;Golovchenko M;Rudenko N;Munderloh U;Hovius JW;Booth CJ;Jacobs-Wagner C;Palm NW;Ring AM;Fikrig E
通讯作者：
Fikrig E
DOI：
10.1073/pnas.0805309105
发表时间：
2008-12-09
影响因子：
11.1
作者：
Huang, Kerwyn Casey;Mukhopadhyay, Ranjan;Wingreen, Ned S.
通讯作者：
Wingreen, Ned S.
影响因子：
6.7
作者：
Davis MM;Brock AM;DeHart TG;Boribong BP;Lee K;McClune ME;Chang Y;Cramer N;Liu J;Jones CN;Jutras BL
通讯作者：
Jutras BL
DOI：
10.1073/pnas.91.8.3433
发表时间：
1994-04-12
影响因子：
11.1
作者：
GOLDSTEIN, SF;CHARON, NW;KREILING, JA
通讯作者：
KREILING, JA
影响因子：
3.2
作者：
Brock，Aaron M.;Jutras，Brandon L.
通讯作者：
Jutras，Brandon L.