Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord.
Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord.
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DOI：
10.4103/1673-5374.282260
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发表时间：
2020-11
影响因子：
6.1
通讯作者：
Park HJ
中科院分区：
文献类型：
--
作者：
Oh JY;Hwang TY;Jang JH;Park JY;Ryu Y;Lee H;Park HJ
尽管有许多治疗神经病理性疼痛的努力，但各种药物往往不能满足需要，并且有许多副作用。白云母是一种铝硅酸盐矿物，已有报道具有抗炎作用，但白云母对神经病理性疼痛的疗效尚未被研究。在这里，我们评估了白云母纳米粒是否可以通过控制神经性疼痛中观察到的炎症过程来减轻疼痛症状。采用神经病理性疼痛的部分坐骨神经结扎模型，将坐骨神经1/3~1/2的三叉神经分叉与背侧紧密捆绑，观察白云母纳米粒的镇痛作用。用白云母纳米粒(4 mg/100μL)肌肉注射治疗神经病理性疼痛(每周3天，连用4周)。结果表明，白云母纳米粒注射剂可明显减轻部分坐骨神经结扎所致的机械性和寒性痛觉超敏。在脊髓中，白云母纳米粒注射对星形胶质细胞和小胶质细胞的激活有抑制作用，并降低了部分坐骨神经结扎模型中上调的促炎细胞因子IL-1β、肿瘤坏死因子-α、白介素6和单核细胞趋化蛋白-1的表达。此外，注射白云母纳米颗粒导致坐骨神经中激活的转录因子3减少，这是一种神经元损伤标志物。这些结果提示，白云母纳米粒对部分坐骨神经结扎诱导的神经病理性疼痛的镇痛作用可能是通过抑制星形胶质细胞和小胶质细胞的激活以及促炎细胞因子来实现的。我们认为，白云母纳米粒是一种潜在的神经病理性疼痛的抗伤害性候选药物。本研究中的所有实验方案于2017年9月28日获得韩国东国大学机构动物伦理委员会(IACUC)的批准(批准号2017-022-1)。
Despite numerous efforts to overcome neuropathic pain, various pharmacological drugs often fail to meet the needs and have many side effects. Muscovite is an aluminosilicate mineral that has been reported to have an anti-inflammatory effect, but the efficacy of muscovite for neuropathic pain has not been investigated. Here, we assessed whether muscovite nanoparticles can reduce the symptoms of pain by controlling the inflammatory process observed in neuropathic pain. The analgesic effects of muscovite nanoparticles were explored using partial sciatic nerve ligation model of neuropathic pain, in which one-third to one-half of the nerve trifurcation of the sciatic nerve was tightly tied to the dorsal side. Muscovite nanoparticles (4 mg/100 μL) was given intramuscularly to evaluate its effects on neuropathic pain (3 days per week for 4 weeks). The results showed that the muscovite nanoparticle injections significantly alleviated partial sciatic nerve ligation-induced mechanical and cold allodynia. In the spinal cord, the muscovite nanoparticle injections exhibited inhibitory effects on astrocyte and microglia activation and reduced the expression of pro-inflammatory cytokines, such as interleukin-1β, tumor necrosis factor-α, interleiukin-6 and monocyte chemoattractant protein-1, which were upregulated in the partial sciatic nerve ligation model. Moreover, the muscovite nanoparticle injections resulted in a decrease in activating transcription factor 3, a neuronal injury marker, in the sciatic nerve. These results suggest that the analgesic effects of muscovite nanoparticle on partial sciatic nerve ligation-induced neuropathic pain may result from inhibiting activation of astrocytes and microglia as well as pro-inflammatory cytokines. We propose that muscovite nanoparticle is a potential anti-nociceptive candidate for neuropathic pain. All experimental protocols in this study were approved by the Institutional Animal Ethics Committee (IACUC) at Dongguk University, South Korea (approval No. 2017-022-1) on September 28, 2017.
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影响因子：
4.6
作者：
Jang JH;Park JY;Oh JY;Bae SJ;Jang H;Jeon S;Kim J;Park HJ
通讯作者：
Park HJ
影响因子：
2.7
作者：
Clark AK;Old EA;Malcangio M
通讯作者：
Malcangio M
影响因子：
3.3
作者：
Jung, Bock-Gie;Toan, Nguyen Tat;Lee, Bong-Joo
通讯作者：
Lee, Bong-Joo
神经炎症驱动的慢性疼痛的新兴目标。
影响因子：
120.1
作者：
Ji, Ru-Rong;Xu, Zhen-Zhong;Gao, Yong-Jing
通讯作者：
Gao, Yong-Jing
影响因子：
3.3
作者：
ANDREEV, N;URBAN, L;DRAY, A
通讯作者：
DRAY, A